Abstract：
Mammalian cells have developed and optimized defense mechanisms to prevent or hamper viral infection. The early transcriptional silencing of incoming viral DNAs is one such antiviral strategy and seems to be of fundamental importance, since most cell types silence unintegrated retroviral DNAs. In this chapter, a method for chromatin immunoprecipitation of unintegrated DNA is described. This technique allows investigators to examine histone and co-factor interactions with unintegrated viral DNAs as well as to analyze histone modifications in general or in a kinetic fashion at various time points during viral infection.
摘要：
哺乳动物细胞已经开发并优化了防御机制以防止或阻碍病毒感染。进入的病毒DNA的早期转录沉默是一个这样的抗病毒策略，似乎是至关重要的，因为大多数细胞类型沉默未整合的逆转录病毒DNA。在这一章中,描述了一种用于未整合DNA的染色质免疫沉淀的方法。该技术允许研究人员检查组蛋白和辅因子与未整合的病毒DNA的相互作用，以及在病毒感染期间的不同时间点一般或以动力学方式分析组蛋白修饰。
