关键词: Behaviour Cartilage Malformations Stress Zebrafish larvae

Mesh : Animals Zebrafish / genetics Stress, Physiological MicroRNAs / genetics metabolism Larva / growth & development genetics Gene Expression Regulation, Developmental Cartilage / abnormalities

来  源:   DOI:10.1007/s10695-024-01355-0   PDF(Pubmed)

Abstract:
The effects of stress during early vertebrate development can be especially harmful. Avoiding stressors in fish larvae is essential to ensure the health of adult fish and their reproductive performance and overall production. We examined the consequences of direct exposure to successive acute stressors during early development, including their effects on miR-29a and its targets, survival, hatching and malformation rates, larval behaviour and cartilage and eye development. Our aim was to shed light on the pleiotropic effects of early-induced stress in this vertebrate model species. Our results showed that direct exposure to successive acute stressors during early development significantly upregulated miR-29a and downregulated essential collagen transcripts col2a1a, col6a2 and col11a1a, decreased survival and increased malformation rates (swim bladder, otoliths, cardiac oedema and ocular malformations), promoting higher rates of immobility in larvae. Our results revealed that stress in early stages can induce different eye tissular architecture and cranioencephalic cartilage development alterations. Our research contributes to the understanding of the impact of stressful conditions during the early stages of zebrafish development, serving as a valuable model for vertebrate research. This holds paramount significance in the fields of developmental biology and aquaculture and also highlights miR-29a as a potential molecular marker for assessing novel larval rearing programmes in teleost species.
摘要:
在脊椎动物早期发育过程中应激的影响可能是特别有害的。避免鱼幼虫的应激源对于确保成年鱼的健康及其繁殖性能和整体生产至关重要。我们研究了在早期发育过程中直接暴露于连续急性应激源的后果,包括它们对miR-29a及其靶标的影响,生存,孵化率和畸形率,幼虫行为和软骨和眼睛发育。我们的目的是阐明这种脊椎动物模型物种中早期诱导应激的多效性作用。我们的结果表明,在早期发育过程中直接暴露于连续的急性应激源显着上调miR-29a和下调必需胶原转录本col2a1a,col6a2和col11a1a,存活率降低和畸形率增加(游泳膀胱,耳石,心脏水肿和眼部畸形),促进幼虫更高的不动率。我们的结果表明,早期阶段的压力可以诱导不同的眼组织结构和颅脑软骨发育改变。我们的研究有助于了解斑马鱼发育早期阶段压力条件的影响,作为脊椎动物研究的有价值的模型。这在发育生物学和水产养殖领域具有至关重要的意义,并且还强调了miR-29a作为评估硬骨鱼物种新型幼虫饲养计划的潜在分子标记。
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