Abstract：
The effect of the parental environment on offspring through non-DNA sequence-based mechanisms, such as DNA methylation, chromatin modifications, noncoding RNAs, and proteins, could only be established after the conception of \"epigenetics\". These effects are now broadly referred to as multigenerational epigenetic effects. Despite accumulating evidence of male gamete-mediated multigenerational epigenetic inheritance, little is known about the factors that underlie heat stress-induced multigenerational epigenetic inheritance via the male germline in Drosophila. In this study, we address this gap by utilizing an established heat stress paradigm in Drosophila and investigating its multigenerational effect on the sperm proteome. Our findings indicate that multigenerational heat stress during the early embryonic stage significantly influences proteins in the sperm associated with translation, chromatin organization, microtubule-based processes, and the generation of metabolites and energy. Assessment of life-history traits revealed that reproductive fitness and stress tolerance remained unaffected by multigenerational heat stress. Our study offers initial insights into the chromatin-based epigenetic mechanisms as a plausible means of transmitting heat stress memory through the male germline in Drosophila. Furthermore, it sheds light on the repercussions of early embryonic heat stress on male reproductive potential. The data sets from this study are available at the ProteomeXchange Consortium under the identifier PXD037488.
摘要：
父母环境通过非DNA序列机制对后代的影响，比如DNA甲基化，染色质修饰，非编码RNA，和蛋白质，只有在“表观遗传学”的概念之后才能建立。这些效应现在被广泛地称为多代表观遗传效应。尽管积累了雄性配子介导的多代表观遗传的证据，关于果蝇中通过雄性种系引起热应激诱导的多代表观遗传的因素知之甚少。在这项研究中,我们通过利用果蝇中已建立的热应激范式并研究其对精子蛋白质组的多代影响来解决这一差距。我们的发现表明，胚胎早期阶段的多代热应激显着影响精子中与翻译相关的蛋白质，染色质组织，基于微管的过程，代谢产物和能量的产生。对生活史特征的评估表明，生殖适应性和应激耐受性不受多代热应激的影响。我们的研究提供了对基于染色质的表观遗传机制的初步见解，作为通过果蝇中雄性种系传递热应激记忆的合理手段。此外，它揭示了早期胚胎热应激对男性生殖潜能的影响。来自本研究的数据集可在ProteomeXchangeConsortium以标识符PXD037488获得。
