Abstract：
BACKGROUND: Bombyx mori nuclear polyhedrosis virus (BmNPV), as a typical baculovirus, is the primary pathogen that infects the silkworm B. mori, a lepidopteran species. Owing to the high biological safety of BmNPV in infecting insects, it is commonly utilized as a biological insecticide for pest control. Apoptosis is important in the interaction between the host and pathogenic microorganisms. MicroRNAs (miRNAs) influence immune responses and promote stability of the immune system via apoptosis. Therefore, the study of apoptosis-related miRNA in silkworms during virus infection can not only provide support for standardizing the prevention and control of diseases and insect pests, but also reduce the economic losses to sericulture caused by the misuse of biological pesticides.
RESULTS: Through transcriptome sequencing, we identified a miRNA, miR-31-5p, and demonstrated that it can inhibit apoptosis in silkworm cells and promote the proliferation of BmNPV in BmE-SWU1 cells. We identified a target gene of miR-31-5p, B. mori cytochrome P450 9e2 (BmCYP9e2), and demonstrated that it can promote apoptosis in silkworm cells and inhibit the proliferation of BmNPV. Moreover, we constructed transgenic silkworm strains with miR-31-5p knockout and confirmed that they can inhibit the proliferation of BmNPV.
CONCLUSIONS: These data indicate that miR-31-5p may exert functions of inhibiting apoptosis and promoting virus proliferation by regulating BmCYP9e2. The findings demonstrate how miRNAs influence host cell apoptosis and how they are involved in the host immune system response to viruses, providing important insights into the applications of biological insecticides for pest control. © 2024 Society of Chemical Industry.
RESULTS: Through transcriptome sequencing, we identified a miRNA, miR-31-5p, and demonstrated that it can inhibit apoptosis in silkworm cells and promote the proliferation of BmNPV in BmE-SWU1 cells. We identified a target gene of miR-31-5p, B. mori cytochrome P450 9e2 (BmCYP9e2), and demonstrated that it can promote apoptosis in silkworm cells and inhibit the proliferation of BmNPV. Moreover, we constructed transgenic silkworm strains with miR-31-5p knockout and confirmed that they can inhibit the proliferation of BmNPV.
CONCLUSIONS: These data indicate that miR-31-5p may exert functions of inhibiting apoptosis and promoting virus proliferation by regulating BmCYP9e2. The findings demonstrate how miRNAs influence host cell apoptosis and how they are involved in the host immune system response to viruses, providing important insights into the applications of biological insecticides for pest control. © 2024 Society of Chemical Industry.
摘要：
背景：家蚕核型多角体病毒（BmNPV），作为一种典型的杆状病毒,是感染家蚕的主要病原体，鳞翅目物种。由于BmNPV感染昆虫的生物安全性高,它通常用作害虫防治的生物杀虫剂。细胞凋亡重要表现在宿主与病原微生物之间的互相感化。MicroRNAs(miRNAs)通过凋亡影响免疫应答并促进免疫系统的稳定性。因此,通过对家蚕病毒感染过程中细胞凋亡相关miRNA的研究,不仅可以为规范病虫害防治提供支持,同时也减少了生物农药误用给蚕业造成的经济损失。
结果：通过转录组测序，我们发现了一个miRNA,miR-31-5p,并证明它能抑制家蚕细胞凋亡，促进BmE-SWU1细胞BmNPV增殖。我们鉴定了miR-31-5p的靶基因,B.细胞色素P4509e2（BmCYP9e2），并证明其可以促进家蚕细胞凋亡，抑制BmNPV的增殖。此外，我们构建了miR-31-5p敲除的转基因家蚕菌株，并证实它们可以抑制BmNPV的增殖。
结论：这些数据表明miR-31-5p可能通过调节BmCYP9e2发挥抑制细胞凋亡和促进病毒增殖的功能。研究结果表明miRNAs如何影响宿主细胞凋亡，以及它们如何参与宿主免疫系统对病毒的反应。为生物杀虫剂在病虫害防治中的应用提供重要见解。©2024化学工业学会。
结果：通过转录组测序，我们发现了一个miRNA,miR-31-5p,并证明它能抑制家蚕细胞凋亡，促进BmE-SWU1细胞BmNPV增殖。我们鉴定了miR-31-5p的靶基因,B.细胞色素P4509e2（BmCYP9e2），并证明其可以促进家蚕细胞凋亡，抑制BmNPV的增殖。此外，我们构建了miR-31-5p敲除的转基因家蚕菌株，并证实它们可以抑制BmNPV的增殖。
结论：这些数据表明miR-31-5p可能通过调节BmCYP9e2发挥抑制细胞凋亡和促进病毒增殖的功能。研究结果表明miRNAs如何影响宿主细胞凋亡，以及它们如何参与宿主免疫系统对病毒的反应。为生物杀虫剂在病虫害防治中的应用提供重要见解。©2024化学工业学会。
