RESULTS: Through transcriptome sequencing, we identified a miRNA, miR-31-5p, and demonstrated that it can inhibit apoptosis in silkworm cells and promote the proliferation of BmNPV in BmE-SWU1 cells. We identified a target gene of miR-31-5p, B. mori cytochrome P450 9e2 (BmCYP9e2), and demonstrated that it can promote apoptosis in silkworm cells and inhibit the proliferation of BmNPV. Moreover, we constructed transgenic silkworm strains with miR-31-5p knockout and confirmed that they can inhibit the proliferation of BmNPV.
CONCLUSIONS: These data indicate that miR-31-5p may exert functions of inhibiting apoptosis and promoting virus proliferation by regulating BmCYP9e2. The findings demonstrate how miRNAs influence host cell apoptosis and how they are involved in the host immune system response to viruses, providing important insights into the applications of biological insecticides for pest control. © 2024 Society of Chemical Industry.
结果:通过转录组测序,我们发现了一个miRNA,miR-31-5p,并证明它能抑制家蚕细胞凋亡,促进BmE-SWU1细胞BmNPV增殖。我们鉴定了miR-31-5p的靶基因,B.细胞色素P4509e2(BmCYP9e2),并证明其可以促进家蚕细胞凋亡,抑制BmNPV的增殖。此外,我们构建了miR-31-5p敲除的转基因家蚕菌株,并证实它们可以抑制BmNPV的增殖。
结论:这些数据表明miR-31-5p可能通过调节BmCYP9e2发挥抑制细胞凋亡和促进病毒增殖的功能。研究结果表明miRNAs如何影响宿主细胞凋亡,以及它们如何参与宿主免疫系统对病毒的反应。为生物杀虫剂在病虫害防治中的应用提供重要见解。©2024化学工业学会。