PDF(Pubmed)
Abstract：
Imaging mass cytometry (IMC) is a metal mass spectrometry-based method allowing highly multiplex immunophenotyping of cells within tissue samples. However, some limitations of IMC are its 1-µm resolution and its time and costs of analysis limiting respectively the detailed histopathological analysis of IMC-produced images and its application to small selected tissue regions of interest (ROI) of one to few square millimeters. Coupling on a single-tissue section, IMC and histopathological analyses could permit a better selection of the ROI for IMC analysis as well as co-analysis of immunophenotyping and histopathological data until the single-cell level. The development of this method is the aim of the present study in which we point to the feasibility of applying the IMC process to tissue sections previously Alcian blue-stained and digitalized before IMC tissue destructive analyses. This method could help to improve the process of IMC in terms of ROI selection, time of analysis, and the confrontation between histopathological and immunophenotypic data of cells.
摘要：
成像质量细胞术(IMC)是一种基于金属质谱的方法,其允许对组织样品内的细胞进行高度多重免疫分型。然而,IMC的一些局限性是其1-µm分辨率以及分析时间和分析成本分别限制了IMC产生的图像的详细组织病理学分析及其对一到几平方毫米的小选定感兴趣组织区域（ROI）的应用。在单组织切片上耦合，IMC和组织病理学分析可以允许更好地选择ROI以进行IMC分析以及免疫表型和组织病理学数据的共同分析，直到单细胞水平。该方法的开发是本研究的目的，在该研究中，我们指出了将IMC过程应用于先前在IMC组织破坏性分析之前进行Alcian蓝色染色和数字化的组织切片的可行性。这种方法可以帮助改善IMC在ROI选择方面的过程，分析时间,以及细胞的组织病理学和免疫表型数据之间的对抗。
