PDF(Pubmed)
Abstract：
Despite the significant health risks associated with Dermanyssus gallinae infestations in humans, they are often overlooked. This study investigated a household case of D. gallinae infestation and explored the resulting clinical manifestations and risk of infection in family members. Microfluidic PCR was employed for high-throughput screening of pathogens in collected mites and blood samples from both chickens and family members. Morphological and molecular examinations confirmed the identity of the mites as D. gallinae sensu stricto (s.s.), with evidence indicating recent blood feeding. Results indicated that the mites exclusively harbored various pathogens, including Bartonella spp., Ehrlichia spp., Apicomplexa, and Theileria spp. Blood samples from family members and poultry tested negative for these pathogens, suggesting a potential reservoir role for D. gallinae. The study further identified haplotypes of D. gallinae, classifying them into D. gallinae s.s., cosmopolitan haplogroup A. Serological analysis revealed elevated IgE seroreactivity against mite proteins in the family member with bite lesions. Antibodies against Bartonella spp. were detected in this individual, indicating exposure to the pathogen. In summary, this study sheds light on the clinical manifestations, pathogen detection, and genetic characterization of D. gallinae infestations, underscoring the necessity of adopting comprehensive approaches to manage such infestations effectively.
摘要：
尽管与人类皮氏真菌感染相关的重大健康风险，他们经常被忽视。这项研究调查了一例家庭感染D.gallinae感染的病例，并探讨了其导致的临床表现和家庭成员感染的风险。微流体PCR用于从鸡和家庭成员收集的螨虫和血液样品中的病原体的高通量筛选。形态学和分子检查证实了螨虫的身份为D.gallinaesensustricto（s.s.），有证据表明最近的血液喂养。结果表明，螨虫仅携带各种病原体，包括巴尔通菌属。,埃里希菌属。,Apicomplex,和Theileriaspp.家庭成员和家禽的血液样本对这些病原体测试呈阴性，暗示了D.gallinae潜在的储层作用。该研究进一步确定了D.gallinae的单倍型，将它们分类为D.gallinaes.s.，世界性单倍群A.血清学分析显示，在有咬伤的家族成员中，IgE对螨蛋白的血清反应性升高。针对巴尔通体的抗体。在这个人中被检测到，表明暴露于病原体。总之,这项研究揭示了临床表现，病原体检测，和D.gallinae感染的遗传特征，强调必须采取全面的方法来有效地管理这种侵扰。
