Abstract：
Symbiotic nitrogen fixation (SNF) is crucial for legumes, providing them with the nitrogen necessary for plant growth and development. Nodulation is the first step in the establishment of SNF. However, the determinant genes in soybean nodulation and the understanding of the underlying molecular mechanisms governing nodulation are still limited. Herein, we identified a phosphatase, GmPP2C61A, which was specifically induced by rhizobia inoculation. Using transgenic hairy roots harboring GmPP2C61A::GUS, we showed that GmPP2C61A was mainly induced in epidermal cells following rhizobia inoculation. Functional analysis revealed that knockdown or knock-out of GmPP2C61A significantly reduced the number of nodules, while overexpression of GmPP2C61A promoted nodule formation. Additionally, GmPP2C61A protein was mainly localized in the cytoplasm and exhibited conserved phosphatase activity in vitro. Our findings suggest that phosphatase GmPP2C61A serves as a critical regulator in soybean nodulation, highlighting its potential significance in enhancing symbiotic nitrogen fixation.
摘要：
共生固氮(SNF)对豆类至关重要，为它们提供植物生长和发育所需的氮。结瘤是建立SNF的第一步。然而,大豆结瘤中的决定基因和对控制结瘤的潜在分子机制的理解仍然有限。在这里，我们发现了一种磷酸酶,GmPP2C61A,这是由根瘤菌接种引起的。使用带有GmPP2C61A::GUS的转基因毛状根，结果表明，接种根瘤菌后，GmPP2C61A主要在表皮细胞中诱导。功能分析显示，敲除或敲除GmPP2C61A可显著减少结节的数量，而GmPP2C61A的过表达促进结节形成。此外,GmPP2C61A蛋白主要定位于细胞质中，在体外表现出保守的磷酸酶活性。我们的研究结果表明，磷酸酶GmPP2C61A是大豆结瘤的关键调节因子。强调其在增强共生固氮方面的潜在意义。
