PDF(Pubmed)
Abstract：
Small ruminant lentiviruses (SRLVs) are widespread and infect goats and sheep. Several reports also suggest that SRLVs can infect wild ruminants. The presence of specific antibodies against SRLVs has been identified in wild ruminants from Poland, but no studies have been conducted to detect proviral DNA of SRLVs in these animals. Therefore, the purpose of this study was to examine samples from Polish wild ruminants to determine whether these animals can serve as reservoirs of SRLVs under natural conditions. A total of 314 samples were tested from red deer (n = 255), roe deer (n = 52) and fallow deer (n = 7) using nested real-time PCR. DNA from positive real-time PCR samples was subsequently used to amplify a CA fragment (625 bp) of the gag gene, a 1.2 kb fragment of the pol gene and an LTR-gag fragment. Three samples (0.95%) were positive according to nested real-time PCR using primers and probe specific for CAEV (SRLV group B). All the samples were negative for the primers and probe specific for MVV (SRLV A group). Only SRLV LTR-gag sequences were obtained from two red deer. Phylogenetic analysis revealed that these sequences were more closely related to CAEV than to MVV. Our results revealed that deer can carry SRLV proviral sequences and therefore may play a role in the epidemiology of SRLVs. To our knowledge, this is the first study describing SRLV sequences from red deer.
摘要：
小反刍动物慢病毒(SRLV)广泛存在并感染山羊和绵羊。一些报告还表明SRLV可以感染野生反刍动物。已经在来自波兰的野生反刍动物中发现了针对SRLV的特异性抗体的存在，但尚未进行研究来检测这些动物的SRLV的前病毒DNA。因此,这项研究的目的是检查波兰野生反刍动物的样本，以确定这些动物是否可以在自然条件下用作SRLV的水库。总共测试了314个来自马鹿的样品（n=255），利用巢式实时PCR技术对毛鹿(n=52)和小鹿(n=7)进行检测。来自阳性实时PCR样品的DNA随后用于扩增gag基因的CA片段（625bp），pol基因的1.2kb片段和LTR-gag片段。根据使用对CAEV(SRLV组B)特异性的引物和探针的巢式实时PCR,三个样品(0.95%)为阳性。所有样品对于MVV(SRLVA组)特异性的引物和探针均为阴性。仅从两只马鹿获得SRLVLTR-gag序列。系统发育分析表明，这些序列与CAEV的关系比与MVV的关系更密切。我们的结果表明，鹿可以携带SRLV前病毒序列，因此可能在SRLV的流行病学中起作用。据我们所知,这是第一项描述马鹿SRLV序列的研究。
