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Abstract：
In order to obtain the best mass spectrometry identification results for using the most appropriate methods in clinical practice, we explore the optimal pretreatment methods for different species and morphologies of filamentous fungi. 98 fungal strains were treated with formic acid sandwich method, dispersion method, extraction method, and other methods using a medium element mass spectrometer (EXS3000) as a platform. Each strain had three targets, and the identification rates and confidence differences under different pre-treatment methods were compared to evaluate the identification effects of these methods. The mass spectrometry identification rates of 98 filamentous fungi obtained after pre-treatment with formic acid sandwich method, dispersion method, and extraction method were 85.71%, 82.65%, and 75.51%, respectively. The identification rate of the formic acid sandwich method was significantly higher than the other two methods (P < 0 005) has the best identification ability and the obtained confidence is also higher than the other two methods. The use of formic acid sandwich method for mass spectrometry identification of filamentous fungi can achieve ideal identification results, which is suitable for mass spectrometry identification of filamentous fungi in conventional laboratories.
摘要：
为了获得最佳的质谱鉴定结果，以便在临床实践中使用最合适的方法，我们探索了不同种类和形态的丝状真菌的最佳预处理方法。用甲酸夹心法处理98株真菌，分散方法，提取方法,和其他使用介质元素质谱仪（EXS3000）作为平台的方法。每个菌株都有三个目标，并比较不同预处理方法下的识别率和置信度差异，以评估这些方法的识别效果。对甲酸夹心法预处理后的98株丝状真菌进行质谱鉴定,分散方法，提取方法为85.71%,82.65%,75.51%,分别。甲酸夹心法的识别率明显高于其他两种方法(P<0005),具有最好的识别能力,获得的置信度也高于其他两种方法。利用甲酸夹心法进行丝状真菌的质谱鉴定可以取得理想的鉴定结果,适用于常规实验室中丝状真菌的质谱鉴定。
