PDF(Pubmed)
Abstract：
Type 2 diabetes mellitus (T2DM) is a chronic inflammatory disease that can compromise the functioning of various organs, including the salivary glands (SG). The purinergic system is one of the most important inflammatory pathways in T2DM condition, and P2X7R and P2X4R are the primary purinergic receptors in SG that regulate inflammatory homeostasis. This study aimed to evaluate P2X7R and P2X4R expression, and morphological changes in the submandibular gland (SMG) in T2DM. Twenty-four 5-week-old mice were randomly assigned to control (CON) and diabetes mellitus (DM) groups (n = 12 each). Body weight, diet, and blood glucose levels were monitored weekly. The histomorphology of the SMG and the expression of the P2X7R, and P2X7R was evaluated by immunohistochemistry (IHC) staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) at 11 and 13 weeks of age. Our findings indicate a significant increase in food consumption, body weight, and blood glucose levels in the DM group. Although a significant increase in P2X7R and P2X4R expression was observed in the DM groups, the receptor location remained unchanged. We also observed a significant increase in the acinar area in the DM13w group, and a significant decrease in the ductal area in the DM11w and DM13w groups. Targeting purinergic receptors may offer novel therapeutic methods for diabetic complications.
摘要：
2型糖尿病（T2DM）是一种慢性炎症性疾病，可以损害各种器官的功能，包括唾液腺（SG）。嘌呤能系统是2型糖尿病最重要的炎症通路之一,P2X7R和P2X4R是SG中调节炎症稳态的主要嘌呤能受体。本研究旨在评估P2X7R和P2X4R的表达，2型糖尿病患者颌下腺（SMG）的形态学变化。将24只5周龄小鼠随机分配到对照组(CON)和糖尿病(DM)组(每组n=12)。体重,饮食，每周监测血糖水平。SMG的组织形态学和P2X7R的表达，和P2X7R在11和13周龄时通过免疫组织化学（IHC）染色和逆转录定量聚合酶链反应（RT-qPCR）进行评估。我们的发现表明食物消费显著增加，体重,和DM组的血糖水平。尽管在DM组中观察到P2X7R和P2X4R表达的显着增加，受体位置保持不变。我们还观察到DM13w组腺泡面积显著增加，DM11w和DM13w组的导管面积显著减少。靶向嘌呤受体可能为糖尿病并发症提供新的治疗方法。
