Abstract：
A novel composite hydrogel was synthesized via Schiff base reaction between chitosan and di-functional poly(ethylene glycol) (DF-PEG), incorporating glucose oxidase (GOx) and cobalt metal-organic frameworks (Co-MOF). The resulting CS/PEG/GOx@Co-MOF composite hydrogel was characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and energy-dispersive X-ray spectroscopy (EDS). The results confirmed successful integration and uniform distribution of Co-MOF within the hydrogel matrix. Functionally, the hydrogel exploits the catalytic decomposition of glucose by GOx to generate gluconic acid and hydrogen peroxide (H2O2), while Co-MOF gradually releases metal ions and protects GOx. This synergy enhanced the antibacterial activity of the composite hydrogel against both Gram-positive (S. aureus) and Gram-negative bacteria (E. coli), outperforming conventional chitosan-based hydrogels. The potential of the composite hydrogel in treating wound infections was evaluated through antibacterial and wound healing experiments. Overall, CS/PEG/GOx@Co-MOF hydrogel holds great promise for the treatment of wound infections, paving the way for further research and potential clinical applications.
摘要：
通过壳聚糖与双官能聚乙二醇(DF-PEG)的希夫碱反应合成了一种新型复合水凝胶,结合葡萄糖氧化酶(GOx)和钴金属有机骨架(Co-MOF)。使用傅里叶变换红外光谱(FTIR)对所得CS/PEG/GOx@Co-MOF复合水凝胶进行表征,扫描电子显微镜（SEM），X射线光电子能谱(XPS),热重分析（TGA），和能量色散X射线光谱（EDS）。结果证实Co-MOF在水凝胶基质内的成功整合和均匀分布。功能上,水凝胶利用GOx对葡萄糖的催化分解产生葡萄糖酸和过氧化氢（H2O2），而Co-MOF逐渐释放金属离子并保护GOx。这种协同作用增强了复合水凝胶对革兰氏阳性（S.金黄色葡萄球菌）和革兰氏阴性菌（E.大肠杆菌)，优于传统的壳聚糖基水凝胶。通过抗菌和伤口愈合实验评估了复合水凝胶治疗伤口感染的潜力。总的来说,CS/PEG/GOx@Co-MOF水凝胶对伤口感染的治疗大有希望，为进一步的研究和潜在的临床应用铺平了道路。
