Abstract:
Nucleotides are important components and the main indicators for judging Cordyceps quality. In this paper, the mixed fermentation process of Schisandra chinensis and Cordyceps tenuipes was systematically studied, and it was proposed that the fermentation products aqueous extract (S-ZAE) had antioxidant activity and anti-AChE ability. Herein, the results of a single factor showed that S. chinensis, yeast extract, inoculum amount, and pH had significant effects on nucleotide synthesis. The fermentation process optimization results were 3% glucose, 0.25% KH2PO4, 2.1% yeast extract, and S. chinensis 0.49% (m/v), the optimal fermentation conditions were 25℃, inoculum 5.8% (v/v), pH 3.8, 6 d. The yield of total nucleotides in the scale-up culture was 0.64 ± 0.027 mg/mL, which was 10.6 times higher than before optimization. S-ZAE has good antioxidant and anti-AChE activities (IC50 0.50 ± 0.050 mg/mL). This fermentation method has the advantage of industrialization, and its fermentation products have the potential to become good functional foods or natural therapeutic agents.
摘要:
核苷酸是重要成分,是判断虫草质量的主要指标。在本文中,系统研究了五味子和冬虫夏草的混合发酵工艺,结果表明,发酵产物水提物(S-ZAE)具有抗氧化活性和抗AChE能力。在这里,单因素结果表明,酵母提取物,接种量,pH对核苷酸合成有显著影响。发酵工艺优化成果为3%葡萄糖,0.25%KH2PO4,2.1%酵母提取物,和南芥0.49%(m/v),最佳发酵条件为25℃,接种物5.8%(v/v),pH3.8,6d。放大培养物中总核苷酸的产量为0.64±0.027mg/mL,比优化前提高了10.6倍。S-ZAE具有良好的抗氧化和抗AChE活性(IC500.50±0.050mg/mL)。这种发酵方法具有工业化的优势,及其发酵产物有可能成为良好的功能性食品或天然治疗剂。