Abstract：
Mosquito coil is commonly used in many African households for protection against mosquito bites. The coil usually has semi-volatile pyrethroids as an active ingredient, which usually diffuse across open space, and the cloud either kills mosquitoes that are exposed, or mosquitoes can be exposed to sublethal doses of the insecticides. This study was conducted to assess the impact of sublethal doses of mosquito coil on the development of insecticide resistance in Aedes aegypti, a major vector for dengue fever and several other arboviral diseases. A laboratory colony of Ae. aegypti was exposed to sublethal doses of a meperfluthrin-based mosquito coil in a Peet-Grady chamber once per generation for 16 generations. The susceptibility of the exposed colony to a diagnostic dose of the mosquito coil as well as to three other insecticides was determined. Three different kdr mutations and five enzyme activities were evaluated in both the exposed and control colonies. After 16 generations of sublethal exposure to mosquito coils, the full diagnostic dose of the coil caused 68% mortality to the exposed colony compared to 100% mortality in the control colony. Mortality caused by deltamethrin (0.05%) was also significantly lower in the exposed colony. The frequency of 1016I kdr mutation as well as MFO and alpha esterase activities were higher in the exposed colony compared to the control colony. This study provides evidence of the development of pyrethroid resistance in an Ae. aegypti population due to sublethal exposure to mosquito coil for 16 generations. Given the large-scale use of mosquito coils in many African households, its role as a pyrethroid resistance selection source should be taken into consideration when designing resistance management strategies.
摘要：
蚊帐在许多非洲家庭中通常用于防止蚊虫叮咬。线圈通常具有半挥发性拟除虫菊酯作为活性成分，通常在开放空间中扩散，云要么杀死暴露的蚊子，或蚊子可以暴露于亚致死剂量的杀虫剂。进行这项研究是为了评估亚致死剂量的蚊香对埃及伊蚊抗药性发展的影响，登革热和其他几种虫媒病毒病的主要媒介。Ae的实验室菌落。埃及伊蚊在Peet-Grady室中每代一次暴露于亚致死剂量的基于甲氟氰菊酯的蚊香，持续16代。确定了暴露的菌落对诊断剂量的蚊香以及其他三种杀虫剂的敏感性。在暴露和对照菌落中评估了三种不同的kdr突变和五种酶活性。在16代亚致死性接触蚊香后，线圈的全部诊断剂量导致暴露菌落68%的死亡率,而对照菌落100%的死亡率.溴氰菊酯引起的死亡率（0.05％）在暴露的菌落中也显着降低。与对照菌落相比，暴露菌落中1016Ikdr突变的频率以及MFO和α酯酶活性更高。这项研究提供了Ae中拟除虫菊酯抗性发展的证据。由于亚致命性接触蚊香16代，埃及伊蚊种群。鉴于许多非洲家庭大量使用蚊香，在设计抗性管理策略时，应考虑其作为拟除虫菊酯抗性选择源的作用。
