PDF(Pubmed)
Abstract：
Staphylococcus epidermidis is one of the predominant bacterial contaminants in platelet concentrates (PCs), a blood component used to treat bleeding disorders. PCs are a unique niche that triggers biofilm formation, the main pathomechanism of S. epidermidis infections. We performed whole genome sequencing of four S. epidermidis strains isolated from skin of healthy human volunteers (AZ22 and AZ39) and contaminated PCs (ST10002 and ST11003) to unravel phylogenetic relationships and decipher virulence mechanisms compared to 24 complete S. epidermidis genomes in GenBank. AZ39 and ST11003 formed a separate unique lineage with strains 14.1 .R1 and SE95, while AZ22 formed a cluster with 1457 and ST10002 closely grouped with FDAAGOS_161. The four isolates were assigned to sequence types ST1175, ST1174, ST73 and ST16, respectively. All four genomes exhibited biofilm-associated genes ebh, ebp, sdrG, sdrH and atl. Additionally, AZ22 had sdrF and aap, whereas ST10002 had aap and icaABCDR. Notably, AZ39 possesses truncated ebh and sdrG and harbours a toxin-encoding gene. All isolates carry multiple antibiotic resistance genes conferring resistance to fosfomycin (fosB), β-lactams (blaZ) and fluoroquinolones (norA). This study reveales a unique lineage for S. epidermidis and provides insight into the genetic basis of virulence and antibiotic resistance in transfusion-associated S. epidermidis strains.
摘要：
表皮葡萄球菌是血小板浓缩物（PC）中的主要细菌污染物之一，用于治疗出血性疾病的血液成分。PC是触发生物膜形成的独特利基，表皮葡萄球菌感染的主要病理机制。我们对从健康人类志愿者（AZ22和AZ39）和受污染的PC（ST10002和ST11003）的皮肤中分离出的四种表皮葡萄球菌菌株进行了全基因组测序，以与GenBank中24个完整的表皮葡萄球菌基因组相比，揭示系统发育关系和破译毒力机制。AZ39和ST11003与菌株14.1形成了单独的独特谱系。R1和SE95，而AZ22与1457和ST10002形成了一个簇，与FDAAGOS_161紧密组合。将四个分离株分别分配给序列类型ST1175、ST1174、ST73和ST16。所有四个基因组都表现出生物膜相关基因ebh，ebp,sdrG,sdrH和atl。此外,AZ22有sdrF和aap，而ST10002有aap和icaABCDR。值得注意的是,AZ39具有截短的ebh和sdrG，并带有毒素编码基因。所有分离株都携带多种抗生素抗性基因，赋予对磷霉素（fosB）的抗性，β-内酰胺(blaZ)和氟喹诺酮(norA)。这项研究揭示了表皮葡萄球菌的独特谱系，并提供了对输血相关表皮葡萄球菌毒力和抗生素抗性的遗传基础的见解。
