PDF(Pubmed)
Abstract：
Intensive poultry farming faces challenges like gut inflammation in the absence of antibiotics, resulting in reduced productivity, heightened susceptibility to enteric diseases, and other complications. Alternative strategies are needed to manage inflammation and maintain sustainable poultry production. Yaks living in high-altitude hypoxic environments have specialized gut microbes. However, yak probiotics remain largely uncharacterized. We previously isolated a strain of Bacillus pumilus (named TS2) from yaks and demonstrated its potential as a probiotic in vitro. Therefore, in this study, we evaluated the in vivo growth-promoting, antioxidant, immune, and anti-inflammatory effects of Bacillus pumilus isolated from yaks in broilers. We demonstrated the safety of TS2 isolated from yaks in broilers. Furthermore, we found that TS2 increased the average daily weight gain (ADWG) and reduced the feed conversion ratio (FCR). Supplementation with TS2 also improved the mucosal morphology, the ratio of villi to crypt cells, and enzyme activity. High-throughput sequencing showed that the abundance of Lactobacillus was higher in the TS2 treated broilers. Importantly, the serum level of malondialdehyde (MDA) was reduced and the levels of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity were increased in the low-dose TS2 group, while the inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were downregulated compared with the control group. We demonstrated that TS2 supplementation can increase the overall growth performance and ameliorate the blood parameters related to inflammation and immunity in broilers.
摘要：
在没有抗生素的情况下，集约化家禽养殖面临着肠道炎症等挑战，导致生产力下降，对肠道疾病的易感性增加，和其他并发症。需要替代策略来控制炎症并维持可持续的家禽生产。生活在高海拔低氧环境中的牦牛具有专门的肠道微生物。然而,牦牛益生菌在很大程度上仍未表征。我们先前从牦牛中分离出一株短小芽孢杆菌（命名为TS2），并证明了其作为体外益生菌的潜力。因此,在这项研究中，我们评估了体内生长促进，抗氧化剂，免疫，牦牛分离的短小芽孢杆菌对肉鸡的抗炎作用。我们证明了从牦牛中分离的TS2在肉鸡中的安全性。此外，我们发现TS2增加了平均日增重（ADWG）并降低了饲料转化率（FCR）。补充TS2也改善了粘膜形态，绒毛与隐窝细胞的比例，和酶活性。高通量测序表明，TS2处理的肉鸡中乳酸菌的丰度较高。重要的是,低剂量TS2组血清丙二醛(MDA)水平降低,总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性升高,而炎症因子白细胞介素-1β(IL-1β),与对照组相比,白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)表达下调。我们证明，补充TS2可以提高肉仔鸡的整体生长性能并改善与炎症和免疫相关的血液参数。
