PDF(Pubmed)
Abstract：
UNASSIGNED: Head and neck squamous cell carcinoma (HNSCCs) is a common cancer type with a high mortality rate and poor prognosis. Recent studies have focused on the role of immune checkpoints in HNSCC progression and in their potential use as prognostic markers and immunotherapeutic candidates. Some immune checkpoints, such as PD-1 and PD-L1, have been studied thoroughly in HNSCC. Other molecules, such as indoleamine 2,3-dioxygenase 1 (IDO1), have been investigated minimally.
UNASSIGNED: IDO1 expression, prognostic potential, and association with the immune profile of HNSCC were explored using online databases, including GEPIA, UALCAN, TIMER2.0, cBioPortal, and LinkedOmics, which utilize TCGA datasets and are freely available for use. For validation purposes, seven pairs of primary and metastatic HNSCC were immunostained for IDO1.
UNASSIGNED: Our analysis revealed significantly higher expression of IDO1 in HNSCC, especially in HPV+ SCCs compared with healthy control tissue. However, IDO1 expression showed weak to no prognostic potential for overall and disease-free survival in HNSCC. IDO1 expression in HNSCC was positively correlated with several immune-related molecules, including most of the immune checkpoints. Additionally, GO enrichment analysis revealed that several immune-related pathways are positively correlated with IDO1 expression in HNSCC, such as response to type I interferon and lymphocyte-mediated immunity pathways. Finally, IDO1 expression positively correlated with infiltration of most of the immune cells in HNSCC, such as CD4+ T cells, CD8+ T cells, M1 and M2 macrophages, dendritic cells, and B cells.
UNASSIGNED: IDO1 expression is closely correlated with the immune profile of the HNSCC. This observation should be explored further to elucidate the potential of targeting IDO1 as a novel immunotherapeutic approach for HNSCC.
UNASSIGNED: IDO1 expression, prognostic potential, and association with the immune profile of HNSCC were explored using online databases, including GEPIA, UALCAN, TIMER2.0, cBioPortal, and LinkedOmics, which utilize TCGA datasets and are freely available for use. For validation purposes, seven pairs of primary and metastatic HNSCC were immunostained for IDO1.
UNASSIGNED: Our analysis revealed significantly higher expression of IDO1 in HNSCC, especially in HPV+ SCCs compared with healthy control tissue. However, IDO1 expression showed weak to no prognostic potential for overall and disease-free survival in HNSCC. IDO1 expression in HNSCC was positively correlated with several immune-related molecules, including most of the immune checkpoints. Additionally, GO enrichment analysis revealed that several immune-related pathways are positively correlated with IDO1 expression in HNSCC, such as response to type I interferon and lymphocyte-mediated immunity pathways. Finally, IDO1 expression positively correlated with infiltration of most of the immune cells in HNSCC, such as CD4+ T cells, CD8+ T cells, M1 and M2 macrophages, dendritic cells, and B cells.
UNASSIGNED: IDO1 expression is closely correlated with the immune profile of the HNSCC. This observation should be explored further to elucidate the potential of targeting IDO1 as a novel immunotherapeutic approach for HNSCC.
摘要：
■头颈部鳞状细胞癌（HNSCC）是一种常见的癌症类型，死亡率高，预后差。最近的研究集中在免疫检查点在HNSCC进展中的作用以及它们作为预后标志物和免疫治疗候选物的潜在用途。一些免疫检查点，如PD-1和PD-L1，已在HNSCC中进行了深入研究。其他分子,如吲哚胺2,3-双加氧酶1(IDO1)，已经被最低限度地调查了。
■IDO1表达式，预后潜力，并使用在线数据库探索与HNSCC免疫谱的关联，包括GEPIA,UALCAN,TIMER2.0,cBioPortal,和LinkedOmics，它利用TCGA数据集，可免费使用。出于验证目的，对7对原发性和转移性HNSCC进行IDO1免疫染色。
■我们的分析显示IDO1在HNSCC中的表达明显更高，与健康对照组织相比，尤其是在HPV+SCC中。然而,IDO1表达在HNSCC中显示出整体和无病存活的弱至无预后潜力。HNSCC中IDO1的表达与几种免疫相关分子呈正相关,包括大部分的免疫检查点.此外,GO富集分析显示，HNSCC中几种免疫相关通路与IDO1表达呈正相关，例如对I型干扰素和淋巴细胞介导的免疫途径的反应。最后,IDO1的表达与HNSCC中大多数免疫细胞的浸润呈正相关，如CD4+T细胞，CD8+T细胞，M1和M2巨噬细胞，树突状细胞,B细胞。
■IDO1表达与HNSCC的免疫谱密切相关。应该进一步探索这一观察结果以阐明靶向IDO1作为HNSCC的新型免疫治疗方法的潜力。
■IDO1表达式，预后潜力，并使用在线数据库探索与HNSCC免疫谱的关联，包括GEPIA,UALCAN,TIMER2.0,cBioPortal,和LinkedOmics，它利用TCGA数据集，可免费使用。出于验证目的，对7对原发性和转移性HNSCC进行IDO1免疫染色。
■我们的分析显示IDO1在HNSCC中的表达明显更高，与健康对照组织相比，尤其是在HPV+SCC中。然而,IDO1表达在HNSCC中显示出整体和无病存活的弱至无预后潜力。HNSCC中IDO1的表达与几种免疫相关分子呈正相关,包括大部分的免疫检查点.此外,GO富集分析显示，HNSCC中几种免疫相关通路与IDO1表达呈正相关，例如对I型干扰素和淋巴细胞介导的免疫途径的反应。最后,IDO1的表达与HNSCC中大多数免疫细胞的浸润呈正相关，如CD4+T细胞，CD8+T细胞，M1和M2巨噬细胞，树突状细胞,B细胞。
■IDO1表达与HNSCC的免疫谱密切相关。应该进一步探索这一观察结果以阐明靶向IDO1作为HNSCC的新型免疫治疗方法的潜力。
