PDF(Pubmed)
Abstract：
Wolfberry (Lycium, of the family Solanaceae) has special nutritional benefits due to its valuable metabolites. Here, 16 wolfberry-specific metabolites were identified by comparing the metabolome of wolfberry with those of six species, including maize, rice, wheat, soybean, tomato and grape. The copy numbers of the riboflavin and phenyllactate degradation genes riboflavin kinase (RFK) and phenyllactate UDP-glycosyltransferase (UGT1) were lower in wolfberry than in other species, while the copy number of the phenyllactate synthesis gene hydroxyphenyl-pyruvate reductase (HPPR) was higher in wolfberry, suggesting that the copy number variation of these genes among species may be the main reason for the specific accumulation of riboflavin and phenyllactate in wolfberry. Moreover, the metabolome-based neighbor-joining tree revealed distinct clustering of monocots and dicots, suggesting that metabolites could reflect the evolutionary relationship among those species. Taken together, we identified 16 specific metabolites in wolfberry and provided new insight into the accumulation mechanism of species-specific metabolites at the genomic level.
摘要：
枸杞（枸杞，茄科的）由于其宝贵的代谢物而具有特殊的营养益处。这里,通过比较枸杞与6种枸杞的代谢组，鉴定出16种枸杞特异性代谢产物，包括玉米,大米,小麦,大豆,番茄和葡萄。枸杞中的核黄素和苯乳酸降解基因核黄素激酶（RFK）和苯乳酸UDP-糖基转移酶（UGT1）的拷贝数低于其他物种，而枸杞中苯乳酸合成基因羟苯基丙酮酸还原酶（HPPR）的拷贝数较高，提示这些基因在种间的拷贝数变异可能是枸杞中核黄素和苯丙氨酸特异性积累的主要原因。此外，基于代谢组的邻居连接树显示出单子叶植物和双子叶植物的不同聚类，这表明代谢物可以反映这些物种之间的进化关系。一起来看,我们在枸杞中鉴定出16种特定代谢产物，并在基因组水平上对物种特异性代谢产物的积累机制提供了新的见解。
