关键词: Degrading enzymes Explorative genomics Genome mining Hortaea werneckii Polyurethane (PU)

Mesh : Polyurethanes Genome, Fungal Whole Genome Sequencing Saccharomycetales / genetics Polyesters / metabolism Geologic Sediments / microbiology

来  源:   DOI:10.1016/j.margen.2024.101111

Abstract:
Hortaea werneckii M-3, a black yeast isolated from the marine sediment of the West Pacific, can utilize polyester polyurethane (PU, Impranil DLN) as a sole carbon source. Here, we present the complete genome of Hortaea werneckii M-3 with the focus on PU degradation enzymes. The total genome size is 38,167,921 bp, consisting of 186 contigs with a N50 length of 651,266 bp and a GC content of 53.06%. Genome annotation analysis predicts a total of 13,462 coding genes, which include 99 tRNAs and 105 rRNAs. Some genes encoding PU degrading enzymes including cutinase and urease are identified in this genome. The genome analysis of Hortaea werneckii M-3 will be helpful for further understanding the degradation mechanism of polyester PU by marine yeasts.
摘要:
HortaeawerneckiiM-3,一种从西太平洋海洋沉积物中分离出的黑色酵母,可以利用聚酯聚氨酯(PU,ImpranilDLN)作为唯一的碳源。这里,我们介绍了HortaeawerneckiiM-3的完整基因组,重点是PU降解酶。总基因组大小为38,167,921bp,由186个重叠群组成,N50长度为651,266bp,GC含量为53.06%。基因组注释分析预测了总共13,462个编码基因,其中包括99个tRNA和105个rRNA。在该基因组中鉴定了一些编码PU降解酶(包括角质酶和脲酶)的基因。对八叶M-3的基因组分析将有助于进一步了解海洋酵母对聚酯PU的降解机理。
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