Abstract：
This study describes the in vitro anthelmintic effect of a hydroalcoholic extract (HA-E) and its fractions from Cyrtocarpa procera fruits against Haemonchus contortus eggs and infective larvae. The HA-E was subjected to bipartition using ethyl acetate, which resulted in an aqueous fraction (Aq-F) and an organic fraction (EtOAc-F). The HA-E and both fractions were tested using the egg hatching inhibition assay (EHIA) and the larval mortality test (LMT). Fractionation of the EtOAc-F was achieved using different chromatographic processes, i.e., open glass column and HPLC analysis. Fractionation of the EtOAc-F gave 18 subfractions (C1R1-C1R18), and those that showed the highest yields (C1R15, C1R16, C1R17 and C1R18) were subjected to anthelmintic assays. The HA-E and the EtOAc-F displayed 100% egg hatching inhibition at 3 and 1 mg/mL, respectively, whereas Aq-F exhibited 92.57% EHI at 3 mg/mL. All subfractions tested showed ovicidal effect. Regarding the larval mortality test, HA-E and EtOAc-F exhibited a larvicidal effect higher than 50% at 50 and 30 mg/mL, respectively. The subfractions that showed the highest larval mortality against H. contortus were C1R15 and C1R17, with larval mortalities of 53.57% and 60.23% at 10 mg/mL, respectively. Chemical analysis of these bioactive subfractions (C1R15 and C1R17) revealed the presence of gallic acid, protocatechuic acid, and ellagic acid. This study shows evidence about the ovicidal and larvicidal properties of C. procera fruits that could make these plant products to be considered as a natural potential anthelmintic agents for controlling haemonchosis in goats and sheep.
摘要：
这项研究描述了水醇提取物（HA-E）及其来自Cyrtocarpaprocera果实的部分对hemonchuscontrorttus卵和感染性幼虫的体外驱虫作用。使用乙酸乙酯对HA-E进行双分配，其产生水性级分(Aq-F)和有机级分(EtOAc-F)。使用卵孵化抑制测定(EHIA)和幼虫死亡率测试(LMT)测试HA-E和两个级分。使用不同的色谱方法实现了EtOAc-F的分馏，即，开放式玻璃柱和HPLC分析。分级分离EtOAc-F,得到18个子馏分(C1R1-C1R18),和那些显示最高产量的（C1R15，C1R16，C1R17和C1R18）进行驱虫试验。HA-E和EtOAc-F在3和1mg/mL时显示出100％的卵孵化抑制作用，分别,而Aq-F在3mg/mL时表现出92.57%的EHI。测试的所有亚组分均显示出杀卵作用。关于幼虫死亡率测试，HA-E和EtOAc-F在50和30mg/mL时表现出高于50％的杀幼虫作用，分别。显示针对H.contortus的幼虫死亡率最高的亚组分是C1R15和C1R17，在10mg/mL时幼虫死亡率分别为53.57％和60.23％，分别。这些生物活性亚组分（C1R15和C1R17）的化学分析揭示了没食子酸的存在，原儿茶酸，还有鞣花酸.这项研究显示了有关C.procera果实的杀卵和杀幼虫特性的证据，这些特性可以使这些植物产品被认为是控制山羊和绵羊血吸虫病的天然潜在驱虫药。
