Abstract：
Here, a novel porous microneedle (PMN) device with bilaterally aligned electroosmotic flow (EOF) enabling controllable dual-mode delivery of molecules is developed. The PMNs placed at anode and cathode compartments are modified with anionic poly-2-acrylamido-2-methyl-1-propanesulfonic acid and cationic poly-(3-acrylamidopropyl) trimethylammonium, respectively. The direction of EOF generated by PMN at the cathode compartment is, therefore, reversed from cathode to anode, countering the unwanted cathodal suctioning of interstitial fluid caused by reverse iontophoresis. With the bilateral alignment of EOF, the versatility of the proposed device is evaluated by delivering molecules with different charges and sizes using Franz cell. In addition, a 3D printed probe device is developed to ease practical handling and minimize electrical stimulation by integrating two PMNs in closed proximity. Finally, the performance of the integrated probe device is demonstrated by dual delivery of a variety of molecules (methylene blue, rhodamine B, and fluorescein isothiocyanate-dextran) using pig skin and vaccination using mice with delivered ovalbumin.
摘要：
这里,开发了一种新型多孔微针（PMN）装置，该装置具有双侧对齐的电渗流（EOF），能够实现分子的可控双模式递送。放置在阳极和阴极室的PMN用阴离子PAMPS（聚-2-丙烯酰胺-2-甲基-1-丙磺酸）和阳离子PAPTAC（聚-（3-丙烯酰胺丙基）三甲基铵）改性，分别。PMN在阴极室产生的EOF的方向为,因此,从阴极到阳极相反，对抗由反向离子电渗疗法引起的间质液的不必要的阴极抽吸。随着EOF的双边对齐，通过使用FranzCell递送具有不同电荷和大小的分子来评估所提出的装置的多功能性。此外,开发了一种3D打印探针设备，通过将两个PMN紧密集成在一起，可以简化实际操作并最大程度地减少电刺激，通过双重递送多种分子(亚甲蓝，罗丹明B和FITC-葡聚糖）使用猪皮并使用递送卵清蛋白的小鼠进行疫苗接种。本文受版权保护。保留所有权利。
