Abstract：
Glucose transporter 4 (GLUT4) directly facilitates cellular uptake of glucose and plays a crucial role in mammalian adipose tissue glucose metabolism. In this work, we constructed a cytosensor for sensitive electrochemiluminescence (ECL) detection of GLUT4 in rat adipocytes (RA cells). A carbon nanotube sponge (CNTSP) was selected to fabricate a permeable electrode to overcome the steric hindrance of cells on the electrode. The expression of GLUT4 after treatment with Ganoderma lucidum polysaccharide (GLP) was assessed by analyzing the luminescence emitted from cell-surface ECL probes. Our preliminary results suggest that GLP promote the expression of GLUT4, thereby enhancing the uptake of the fluorescent glucose 2-NBDG. Treatment with GLP affected GLUT4 expression in RA cells in a dose-dependent manner. Additionally, the ECL cytosensor contributes to the development of ECL imaging of receptors on the cell surface for clinical drug evaluation.
摘要：
葡萄糖转运蛋白4（GLUT4）直接促进葡萄糖的细胞摄取，并在哺乳动物脂肪组织葡萄糖代谢中起关键作用。在这项工作中,我们构建了细胞传感器，用于敏感的电化学发光（ECL）检测大鼠脂肪细胞（RA细胞）中的GLUT4。选择碳纳米管海绵(CNTSP)来制造可渗透电极以克服电极上细胞的空间位阻。通过分析细胞表面ECL探针发射的发光来评估用灵芝多糖(GLP)处理后GLUT4的表达。我们的初步结果表明,GLP促进GLUT4的表达,从而增强荧光葡萄糖2-NBDG的摄取。用GLP处理以剂量依赖性方式影响RA细胞中的GLUT4表达。此外,ECL细胞传感器有助于开发用于临床药物评估的细胞表面受体的ECL成像。
