Abstract：
Aedes-transmitted arboviral infections such as Dengue, Yellow Fever, Zika and Chikungunya are increasing public health problems. Xenorhabdus and Photorhabdus bacteria are promising sources of effective compounds with important biological activities. This study investigated the effects of cell-free supernatants of X. szentirmaii, X. cabanillasii and P. kayaii against Ae. aegypti eggs and larvae and identified the bioactive larvicidal compound in X. szentirmaii using The EasyPACId method. Among the three tested bacterial species, X. cabanillasii exhibited the highest (96%) egg hatching inhibition and larvicidal activity (100% mortality), whereas P. kayaii was the least effective species in our study. EasyPACId method revealed that bioactive larvicidal compound in the bacterial supernatant was fabclavine. Fabclavines obtained from promoter exchange mutants of different bacterial species such as X. cabanillasii, X. budapestensis, X. indica, X. szentirmaii, X. hominckii and X. stockiae were effective against mosquito larvae. Results show that these bacterial metabolites have potential to be used in integrated pest management (IPM) programmes of mosquitoes.
摘要：
伊蚊传播的虫媒病毒感染，如登革热，黄热病,寨卡病毒和基孔肯雅病毒正在增加公共卫生问题。横纹肌和光纹肌细菌是具有重要生物活性的有效化合物的有希望的来源。这项研究调查了X.szentirmaii的无细胞上清液的影响，X.卡巴尼拉西和P.kayaii对阵Ae。埃及伊蚊卵和幼虫，并使用easyPACId方法在X.szentirmaii中鉴定了生物活性的杀幼虫化合物。在三个测试的细菌物种中，X.cabanillasii表现出最高的（96％）卵孵化抑制和杀幼虫活性（100％死亡率），而P.kayaii是我们研究中效果最差的物种。EasyPACId方法显示细菌上清液中的生物活性杀幼虫化合物是法克林。从不同细菌物种的启动子交换突变体获得的Fabclavines，例如X.cabanillasii，X.budapestensis,X.indica,X.szentirmaii,X.hominckii和X.stockiae对蚊子幼虫有效。结果表明，这些细菌代谢物有可能用于蚊子的害虫综合治理（IPM）计划。
