Abstract：
This study presents a potent paper-based analytical device (PAD) for quantifying various sugars using an innovative bi-nanozyme made from a 2-dimensional Fe/Ce metal-organic framework (FeCe-BTC). The MOF showed excellent bifunctional peroxidase-oxidase activities, efficiently catalyzing luminol\'s chemiluminescence (CL) reaction. As a peroxidase-like nanozyme, FeCe-BTC could facilitate the dissociation of hydrogen peroxide (H2O2) into hydroxyl radicals, which then oxidize luminol. Additionally, it was also discovered that when reacting with H2O2, the MOF turns into a mixed-valence MOF, and acts as an oxidase nanozyme. This activity is caused by the generated Ce4+ ions in the structure of MOF that can directly oxidize luminol. The MOF was directly synthesized on the PAD and cascaded with specific natural enzymes to establish simple, rapid, and selective CL sensors for the measurement of different sugars. A cell phone was also used to record light intensities, which were then correlated to the analyte concentration. The designed PAD showed a wide linear range of 0.1-10 mM for glucose, fructose, and sucrose, with detection limits of 0.03, 0.04, and 0.04 mM, respectively. It showed satisfactory results in food and biological samples with recovery values ranging from 95.8 to 102.4 %, which makes it a promising candidate for point-of-care (POC) testing for food control and medicinal purposes.
摘要：
这项研究提出了一种有效的基于纸的分析装置（PAD），用于使用由二维Fe/Ce金属有机框架（FeCe-BTC）制成的创新双纳米酶定量各种糖。MOF表现出优异的双功能过氧化物酶-氧化酶活性，高效催化鲁米诺的化学发光(CL)反应。作为过氧化物酶样纳米酶，FeCe-BTC能促进过氧化氢(H2O2)分解为羟基自由基,然后氧化鲁米诺。此外,还发现，当与H2O2反应时，MOF会变成混合价MOF，并充当氧化酶纳米酶。这种活性是由MOF结构中生成的Ce4+离子直接氧化鲁米诺引起的。MOF直接在PAD上合成,并与特定的天然酶级联,快速,和用于测量不同糖的选择性CL传感器。手机也被用来记录光强度，然后将其与分析物浓度相关联。设计的PAD对葡萄糖表现出0.1-10mM的宽线性范围，果糖,和蔗糖，检出限为0.03、0.04和0.04mM，分别。在食品和生物样品中显示出令人满意的结果，回收率为95.8至102.4％，这使其成为用于食品控制和医疗目的的即时护理（POC）测试的有希望的候选人。
