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Abstract：
The role of selenium in the developmental process of esophageal cancer (EC) requires further investigation. To explore the relationship between selenium-related factors and EC through bioinformatic analysis, a case-control study was conducted to verify the results. Utilizing the GEPIA and TCGA databases, we delineated the differential expression of glutathione peroxidase 3 (GPx3) in EC and normal tissues, identified differentially expressed genes (DEGs), and a performed visualization analysis. Additionally, 100 pairs of dietary and plasma samples from esophageal precancerous lesions (EPLs) of esophageal squamous cancer (ESCC) cases and healthy controls from Huai\'an district, Jiangsu, were screened. The levels of dietary selenium, plasma selenium, and related enzymes were analyzed using inductively coupled plasma mass spectrometry (ICP-MS) or ELISA kits. The results showed lower GPx3 expression in tumor tissues compared to normal tissues. Further analysis revealed that DEGs were mainly involved in the fat digestion and absorption pathway, and the core protein fatty acid binding protein 1 (FABP1) was significantly upregulated and negatively correlated with GPx3 expression. Our case-control study found that selenium itself was not associated with EPLs risk. However, both the decreased concentration of GPx3 and the increase in FABP1 were positively correlated with the EPLs risk (p for trend = 0.035 and 0.046, respectively). The different expressions of GPx3 and FABP1 reflect the potential of selenium for preventing ESCC at the EPLs stage. GPx3 may affect myocardial infarction through FABP1, which remains to be further studied.
摘要：
硒在食管癌（EC）发育过程中的作用需要进一步研究。通过生物信息学分析探讨硒相关因子与EC的关系,我们进行了一项病例对照研究以验证结果.利用GEPIA和TCGA数据库，我们描述了谷胱甘肽过氧化物酶3（GPx3）在EC和正常组织中的差异表达，鉴定的差异表达基因（DEGs），和执行的可视化分析。此外,来自淮安地区的食管鳞癌（ESCC）病例和健康对照的100对食道癌前病变（EPLs）的饮食和血浆样本，江苏,被筛选。日粮硒的水平，等离子体硒，使用电感耦合等离子体质谱(ICP-MS)或ELISA试剂盒分析相关酶。结果显示与正常组织相比,肿瘤组织中的GPx3表达较低。进一步分析发现DEGs主要参与脂肪的消化吸收途径,核心蛋白脂肪酸结合蛋白1(FABP1)表达明显上调,与GPx3表达呈负相关。我们的病例对照研究发现，硒本身与EPL风险无关。然而,GPx3浓度降低和FABP1升高均与EPL风险呈正相关(趋势p分别为0.035和0.046).GPx3和FABP1的不同表达反映了硒在EPL阶段预防ESCC的潜力。GPx3可能通过FABP1影响心肌梗死，有待进一步研究。
