PDF(Pubmed)
Abstract：
Acne vulgaris is a prevalent skin disorder affecting many young individuals, marked by keratinization, inflammation, seborrhea, and colonization by Cutibacterium acnes (C. acnes). Ellagitannins, known for their antibacterial and anti-inflammatory properties, have not been widely studied for their anti-acne effects. Chestnut (Castanea sativa Mill., C. sativa), a rich ellagitannin source, including castalagin whose acne-related bioactivity was previously unexplored, was investigated in this study. The research assessed the effect of C. sativa leaf extract and castalagin on human keratinocytes (HaCaT) infected with C. acnes, finding that both inhibited IL-8 and IL-6 release at concentrations below 25 μg/mL. The action mechanism was linked to NF-κB inhibition, without AP-1 involvement. Furthermore, the extract displayed anti-biofilm properties and reduced CK-10 expression, indicating a potential role in mitigating inflammation, bacterial colonization, and keratosis. Castalagin\'s bioactivity mirrored the extract\'s effects, notably in IL-8 inhibition, NF-κB inhibition, and biofilm formation at low μM levels. Other polyphenols, such as flavonol glycosides identified via LC-MS, might also contribute to the extract\'s biological activities. This study is the first to explore ellagitannins\' potential in treating acne, offering insights for developing chestnut-based anti-acne treatments pending future in vivo studies.
摘要：
寻常痤疮是一种流行的皮肤病，影响许多年轻人，以角质化为标志，炎症,皮脂溢出,和粉刺杆菌的定殖(C.痤疮）。Ellagitannins,以其抗菌和抗炎特性而闻名，它们的抗痤疮作用尚未得到广泛研究。栗子（栗子。,C.sativa)，丰富的ellagitannin来源，包括castalagin，其与痤疮相关的生物活性以前未被探索过，在这项研究中进行了调查。该研究评估了紫花苜蓿叶提取物和castalagin对感染痤疮梭菌的人角质形成细胞（HaCaT）的影响。发现在浓度低于25μg/mL时，两者均抑制IL-8和IL-6的释放。其作用机制与NF-κB抑制有关,没有AP-1参与。此外，提取物表现出抗生物膜特性并降低CK-10表达，表明在减轻炎症方面的潜在作用，细菌定植，角化病.Castalagin的生物活性反映了提取物的作用，特别是在IL-8抑制中，NF-κB抑制,和低μM水平的生物膜形成。其他多酚,如通过LC-MS鉴定的黄酮醇苷，也可能有助于提取物的生物活性。这项研究是第一个探索的elligagannins\'治疗痤疮的潜力，为开发基于栗子的抗痤疮治疗提供见解，等待未来的体内研究。
