PDF(Pubmed)
Abstract：
UVB radiation is known to induce photodamage to the skin, disrupt the skin barrier, elicit cutaneous inflammation, and accelerate the aging process. Agaricus blazei Murill (ABM) is an edible medicinal and nutritional fungus. One of its constituents, Agaricus blazei Murill polysaccharide (ABP), has been reported to exhibit antioxidant, anti-inflammatory, anti-tumor, and immunomodulatory effects, which suggests potential effects that protect against photodamage. In this study, a UVB-induced photodamage HaCaT model was established to investigate the potential reparative effects of ABP and its two constituents (A1 and A2). Firstly, two purified polysaccharides, A1 and A2, were obtained by DEAE-52 cellulose column chromatography, and their physical properties and chemical structures were studied. A1 and A2 exhibited a network-like microstructure, with molecular weights of 1.5 × 104 Da and 6.5 × 104 Da, respectively. The effects of A1 and A2 on cell proliferation, the mitochondrial membrane potential, and inflammatory factors were also explored. The results show that A1 and A2 significantly promoted cell proliferation, enhanced the mitochondrial membrane potential, suppressed the expression of inflammatory factors interleukin-1β (IL-1β), interleukin-8 (IL-8), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α), and increased the relative content of filaggrin (FLG) and aquaporin-3 (AQP3). The down-regulated JAK-STAT signaling pathway was found to play a role in the response to photodamage. These findings underscore the potential of ABP to ameliorate UVB-induced skin damage.
摘要：
已知UVB辐射会对皮肤造成光损伤，破坏皮肤屏障，引起皮肤炎症，加速衰老过程。姬松茸（ABM）是一种可食用的药用和营养真菌。它的一个组成部分，姬松茸多糖（ABP），据报道表现出抗氧化剂，抗炎,抗肿瘤，和免疫调节作用，这表明了防止光损伤的潜在影响。在这项研究中,建立了UVB诱导的光损伤HaCaT模型，以研究ABP及其两种成分（A1和A2）的潜在修复作用。首先,两种纯化的多糖，A1和A2，通过DEAE-52纤维素柱层析获得，并对其物理性质和化学结构进行了研究。A1和A2表现出网状微观结构，分子量为1.5×104Da和6.5×104Da，分别。A1和A2对细胞增殖的影响，线粒体膜电位，和炎症因子也进行了探索。结果表明A1和A2显著促进细胞增殖,增强了线粒体膜电位，抑制炎症因子白细胞介素-1β（IL-1β）的表达，白细胞介素-8（IL-8），白细胞介素-6（IL-6），和肿瘤坏死因子α（TNF-α），并增加了聚丝蛋白（FLG）和水通道蛋白3（AQP3）的相对含量。发现下调的JAK-STAT信号通路在对光损伤的响应中起作用。这些发现强调了ABP改善UVB诱导的皮肤损伤的潜力。
