PDF(Pubmed)
Abstract：
In this study, a rutabaga (Brassica napus ssp. napobrassica) donor parent FGRA106, which exhibited broad-spectrum resistance to 17 isolates representing 16 pathotypes of Plasmodiophora brassicae, was used in genetic crosses with the susceptible spring-type canola (B. napus ssp. napus) accession FG769. The F2 plants derived from a clubroot-resistant F1 plant were screened against three P. brassicae isolates representing pathotypes 3A, 3D, and 3H. Chi-square (χ2) goodness-of-fit tests indicated that the F2 plants inherited two major clubroot resistance genes from the CR donor FGRA106. The total RNA from plants resistant (R) and susceptible (S) to each pathotype were pooled and subjected to bulked segregant RNA-sequencing (BSR-Seq). The analysis of gene expression profiles identified 431, 67, and 98 differentially expressed genes (DEGs) between the R and S bulks. The variant calling method indicated a total of 12 (7 major + 5 minor) QTLs across seven chromosomes. The seven major QTLs included: BnaA5P3A.CRX1.1, BnaC1P3H.CRX1.2, and BnaC7P3A.CRX1.1 on chromosomes A05, C01, and C07, respectively; and BnaA8P3D.CRX1.1, BnaA8P3D.RCr91.2/BnaA8P3H.RCr91.2, BnaA8P3H.Crr11.3/BnaA8P3D.Crr11.3, and BnaA8P3D.qBrCR381.4 on chromosome A08. A total of 16 of the DEGs were located in the major QTL regions, 13 of which were on chromosome C07. The molecular data suggested that clubroot resistance in FGRA106 may be controlled by major and minor genes on both the A and C genomes, which are deployed in different combinations to confer resistance to the different isolates. This study provides valuable germplasm for the breeding of clubroot-resistant B. napus cultivars in Western Canada.
摘要：
在这项研究中,大头菜(甘蓝型油菜。napobrassica）供体亲本FGRA106，对代表16种十字形疟原虫致病型的17个分离株表现出广谱抗性，用于易感春季型油菜的遗传杂交(B.napusssp.napus)登录号FG769。针对代表致病性3A的三种十字花菜分离株，筛选了来自根枯病抗性F1植物的F2植物，3D,和3H。卡方（χ2）拟合优度测试表明，F2植物从CR供体FGRA106继承了两个主要的根茎抗性基因。汇集来自对每种病理型具有抗性(R)和易感(S)的植物的总RNA,并进行大量分离体RNA测序(BSR-Seq)。基因表达谱分析确定了R和S块之间的431、67和98个差异表达基因（DEG）。变体调用方法表明，在七个染色体上共有12个（7个主要QTL5个次要QTL）。七个主要的QTL包括:BnaA5P3A。CRX1.1，BnaC1P3H。CRX1.2和BnaC7P3A。CRX1.1分别在染色体A05、C01和C07上;和BnaA8P3D上。CRX1.1，BnaA8P3D。RCr91.2/BnaA8P3H。RCr91.2，BnaA8P3H。Crr11.3/BnaA8P3D。Crr11.3和BnaA8P3D。染色体A08上的qBrCR381.4。共有16个DEGs位于主要的QTL地区，其中在C07染色体上有13。分子数据表明，FGRA106中的根肿病抗性可能受A和C基因组上的主要和次要基因控制。它们以不同的组合部署，以赋予不同的分离株抗性。本研究为加拿大西部抗甘蓝型油菜品种的选育提供了有价值的种质资源。
