关键词: PCR Pakistan Sahiwal cattle casein milk production single nucleotide polymorphism

来  源:   DOI:10.3390/ani14091306   PDF(Pubmed)

Abstract:
The aim of the present study was the validation of the already reported Bos taurus SNPs in the Sahiwal breed. A total of nine SNPs of the casein gene were studied. Out of nine, seven Bos taurus SNPs of casein protein genes were found to be significantly associated with milk productivity traits. The genomic DNA was extracted from the mammary alveolar endothelial cells of a flock of 80 purebred Sahiwal lactating dams available at Khizrabad Farm near Sargodha. New allele-specific primers were designed from the NCBI annotated sequence database of Bos taurus to obtain 100 nt-long PCR products. Each dam was tested separately for all the SNPs investigated. Animals with genotype GG for the SNPs rs43703010, rs10500451, and 110323127, respectively, exhibited high milk yield. Similarly, animals with genotype AA for the SNPs rs11079521, rs43703016, and rs43703017 showed high milk yield consistently. For the SNP rs43703015, animals with genotype CC showed high milk productivity. These above-mentioned SNPs have previously been reported to significantly up-regulate casein protein contents in Bos taurus. Our results indicated SNPs that significantly affect the milk protein contents may also significantly increase per capita milk yield. These finding suggest that the above-mentioned reported SNPs can also be used as genetic markers of milk productivity in Sahiwal cattle.
摘要:
本研究的目的是验证已经报道的Sahiwal品种中的BostaurusSNP。研究了酪蛋白基因的总共9个SNP。九个人中,发现酪蛋白基因的七个BostaurusSNP与牛奶生产率性状显着相关。基因组DNA是从Sargodha附近的Khizrabad农场提供的80只纯种Sahiwal泌乳大坝的蜂群的乳腺肺泡内皮细胞中提取的。从Bostaurus的NCBI注释序列数据库设计新的等位基因特异性引物以获得100nt长的PCR产物。针对所研究的所有SNP分别测试每个大坝。GG基因型分别为rs43703010、rs10500451和110323127的动物,表现出高牛奶产量。同样,SNPsrs11079521、rs43703016和rs43703017基因型AA的动物始终显示出高产奶量。对于SNPrs43703015,具有基因型CC的动物显示出高的奶生产率。先前已报道这些上述SNP显著上调Bos金牛座中的酪蛋白含量。我们的结果表明,显着影响乳蛋白含量的SNP也可能显着提高人均产奶量。这些发现表明,上述报道的SNP也可用作Sahiwal牛的奶生产力的遗传标记。
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