PDF(Pubmed)
Abstract：
The average life of a dog is generally maintained at ten to fifteen years, and tumours are the predominant reason that leads to the death of dogs, especially canine mammary carcinoma. Therefore, early diagnosis of tumours is very important. In this study, tumor size, morphology, and texture could be seen through general clinical examination, tumor metastasis could be seen through imaging examination, inflammatory reactions could be seen through hematological examination, and abnormal cell morphology could be seen through cytological and histopathological examination. In the 269 malignant cases and 179 benign cases, we randomly selected 30 cases each, and an additional 30 healthy dogs were selected for the experiment (healthy dogs: dogs in good physical condition without any tumor or other diseases). We used RT-qPCR and ELISA to determine the relative expression of vascular endothelial growth factor (VEGF), tumor protein P53 (P53), serum ferritin (SF), and NOD-like receptor protein 3 (NLRP3) in 30 healthy dogs, 30 dogs with benign mammary tumours, and 30 dogs with malignant mammary tumours. In the results, the same expression trend was obtained both in serum and tissues, and the expression of the four markers was the highest in malignant mammary tumours, with highly significant differences compared with the benign and healthy/paracancerous groups. By plotting the ROC curves, it was found that the results of combined tests were better than a single test and the combination of the four markers was the best for the early diagnosis. In conclusion, this can assist the clinical early diagnosis to a certain extent, and also provides some references and assistance for the development of tumor detection kits in clinical practice.
摘要：
狗的平均寿命一般维持在十到十五年,肿瘤是导致狗死亡的主要原因，尤其是犬乳腺癌.因此,肿瘤的早期诊断非常重要。在这项研究中,肿瘤大小,形态学,质地可以通过一般临床检查看到，通过影像学检查可以看到肿瘤转移，炎症反应可以通过血液学检查看到，细胞学和组织病理学检查可见细胞形态异常。在269例恶性病例和179例良性病例中，我们随机选择了30例，选择另外30只健康狗进行实验(健康狗:身体状况良好、没有任何肿瘤或其他疾病的狗)。我们使用RT-qPCR和ELISA来确定血管内皮生长因子（VEGF）的相对表达，肿瘤蛋白P53（P53），血清铁蛋白（SF），30只健康犬的NOD样受体蛋白3（NLRP3），30只患有良性乳腺肿瘤的狗，和30只患有恶性乳腺肿瘤的狗。在结果中，在血清和组织中获得相同的表达趋势，这四种标志物在恶性乳腺肿瘤中的表达最高，与良性和健康/癌旁组相比，差异有统计学意义。通过绘制ROC曲线，结果发现，联合测试的结果优于单一测试，四种标志物的组合是早期诊断的最佳选择。总之,这可以在一定程度上帮助临床早期诊断，为临床上肿瘤检测试剂盒的开发提供一定的参考和帮助。
