Abstract：
We report the genomic analysis of a novel alphabaculovirus, Mythimna sequax nucleopolyhedrovirus isolate CNPSo-98 (MyseNPV-CNPSo-98), obtained from cadavers of the winter crop pest, Mythimna sequax Franclemont (Lepidoptera: Noctuidae). The insects were collected from rice fields in Southern Brazil in the 1980\'s and belongs to the \'EMBRAPA-Soja\' Virus Collection. High-throughput sequencing reads of DNA from MyseNPV occlusion bodies and assembly of the data yielded an AT-rich circular genome contig of 148,403 bp in length with 163 annotated opening reading frames (ORFs) and four homologous regions (hrs). Phylogenetic inference based on baculovirus core protein sequence alignments indicated that MyseNPV-CNPSo-98 is a member of Alphabaculovirus genus that clustered with other group II noctuid-infecting baculoviruses, including viruses isolated from Helicoverpa armigera and Mamestra spp. The genomes of the clade share strict collinearity and high pairwise nucleotide identity, with a common set of 149 genes, evolving under negative selection, except a bro gene. Branch lengths and Kimura-2-parameter pairwise nucleotide distances indicated that MyseNPV-CNPSo-98 represents a distinct lineage that may not be classified in any of the currently listed species in the genus.
摘要：
我们报告了一种新的杆状病毒的基因组分析，Mythimnasequax核型多角体病毒分离株CNPSo-98（MyseNPV-CNPSo-98），从冬季作物害虫的尸体中获得，MythimnasequaxFranclemont（鳞翅目：夜蛾科）。这些昆虫是在1980年代从巴西南部的稻田中收集的，属于“EMBRAPA-Soja”病毒收集。来自MyseNPV闭塞体的DNA的高通量测序读取和数据的组装产生了长度为148,403bp的富含AT的环状基因组重叠群，具有带注释的开放阅读框（ORF）和四个同源区域（小时）。基于杆状病毒核心蛋白序列比对的系统发育推断表明，MyseNPV-CNPSo-98是Alphabaculovirus属的成员，与其他II类夜蛾感染杆状病毒成簇，包括从棉铃虫和Mamestraspp中分离出的病毒。进化枝的基因组具有严格的共线性和高的成对核苷酸同一性，有一组共同的149个基因，在否定选择下进化，除了兄弟基因.分支长度和Kimura-2参数成对核苷酸距离表明MyseNPV-CNPSo-98代表了一个不同的谱系，无法在该属中目前列出的任何物种中进行分类。
