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Abstract：
Ultrasound therapy is a method of applying ultrasonic energy to the stimulation produced by human body to change the function and tissue state of the body in order to achieve the purpose of treating diseases. Chronic venous ulcer is a common chronic skin ulcer. GSE222503 for ultrasound therapy of chronic venous ulcers was downloaded from gene expression omnibus database, which were used to identify differentially expressed genes. Weighted gene co-expression network analysis, functional enrichment analysis, gene set enrichment analysis, immune infiltration analysis and construction and analysis of protein-protein interaction network were performed. Draw gene expression heatmaps. Comparative toxicogenomics database analysis was performed. Two hundred thirty-five differentially expressed genes were obtained. According to gene ontology analysis, in biological process analysis, they were mainly enriched in positive regulation of cellular biosynthetic process, reproductive cell development, vasculogenesis, vascular morphogenesis, and inflammatory response. In cellular component analysis, they were mainly enriched in leading edge of growing cell, extracellular matrix binding organelle, F-actin capping protein complex. In molecular function analysis, they were mainly concentrated in receptor ligand activity, cytokine receptor binding. In Kyoto encyclopedia of genes and genomes analysis, they were mainly enriched in cytokine-cytokine receptor interaction, PI3K-Akt signaling pathway, HIF-1 signaling pathway, heme biosynthesis. In weighted gene co-expression network analysis, the soft threshold power was set to 9. Thirty modules were generated. PF4, NR1I2, TTC16, H3C12, KLRB1, CYP21A2 identified by 4 algorithms (MCC, EPC, closeness, stress). Heatmap of core gene expression showed that H3C12, KLRB1, PF4, NR1I2 were all underexpressed in samples of ultrasound-treated chronic venous ulcers and overexpressed in samples of untreated chronic venous ulcers. Comparative toxicogenomics database analysis showed that H3C12, KLRB1, PF4, NR1I2 are associated with thrombophlebitis, phlebitis, vascular malformations, metabolic syndrome, ulcers, and inflammation. In samples of chronic venous ulcer tissue treated with ultrasound, NR1I2 shows low expression, while in samples of chronic venous ulcer tissue without ultrasound treatment, it shows high expression. This finding suggests a potential role of NR1I2 in the process of ultrasound therapy for chronic venous ulcers, which may be related to the therapeutic effect of ultrasound therapy on chronic venous ulcers.
摘要：
超声治疗是将超声能量应用于人体产生的刺激，以改变身体的功能和组织状态，从而达到治疗疾病的目的的方法。慢性静脉性溃疡是一种常见的慢性皮肤溃疡。用于超声治疗慢性静脉性溃疡的GSE222503从基因表达综合数据库下载，用于鉴定差异表达的基因。加权基因共表达网络分析，功能富集分析，基因集富集分析，免疫浸润分析、蛋白质相互作用网络的构建和分析。绘制基因表达热图。进行了比较毒物基因组学数据库分析。获得了235个差异表达基因。根据基因本体论分析,在生物过程分析中，它们主要富含对细胞生物合成过程的正向调节，生殖细胞发育，血管生成,血管形态发生,和炎症反应。在细胞组分分析中，它们主要富集在生长细胞的前缘，细胞外基质结合细胞器，F-肌动蛋白加帽蛋白复合物。在分子功能分析中,它们主要集中在受体配体活性，细胞因子受体结合。在京都基因和基因组分析百科全书中，它们主要富集在细胞因子-细胞因子受体相互作用中，PI3K-Akt信号通路,HIF-1信号通路,血红素生物合成。在加权基因共表达网络分析中，软阈值功率设置为9。生成了30个模块。PF4，NR1I2，TTC16，H3C12，KLRB1，CYP21A2通过4种算法（MCC，EPC,亲密关系,stress).核心基因表达的热图显示，在超声治疗的慢性静脉性溃疡样品中，H3C12，KLRB1，PF4，NR1I2均表达不足，而在未治疗的慢性静脉性溃疡样品中过表达。比较毒物基因组学数据库分析显示，H3C12、KLRB1、PF4、NR1I2与血栓性静脉炎相关，静脉炎,血管畸形,代谢综合征,溃疡,和炎症。在超声治疗的慢性静脉性溃疡组织样本中，NR1I2显示低表达，而在未经超声治疗的慢性静脉性溃疡组织样本中，表现出高表达。这一发现表明NR1I2在慢性静脉性溃疡的超声治疗过程中具有潜在的作用。这可能与超声治疗慢性静脉性溃疡的疗效有关。
