Abstract：
Natural Coral Particles (NCPs) are a suitable scaffold material for Guided Bone Regeneration (GBR) procedures; it combines the placement of a bone substitute supporting a barrier membrane. Due to increasing sea pollution and the declarations of endangered coral species (KYOTO 1997), they are no longer suitable for the medical industry. Novel domestic corals have been grown under controlled conditions to produce cultivated coral graft (CCG) material. This study aimed to evaluate a new CCG in an in vivo experimental GBR procedure. The calvarias of 8 rabbits were surgically exposed, and circular defects 8 mm in diameter were prepared. One defect was filled with CCG particles (experimental group); the contralateral defect (control group) was spontaneously filled by blood clot. The defects were covered with a collagen membrane. Animals were euthanized after 8 weeks. Histological observations of the defects showed similar bone growth patterns in both experimental and control osteotomies. In the experimental defects, no traces of coral particles were observed. Histometric analysis showed denser bone in the pristine zone (65-66%) than in the peripheral zone for both the control (50%) and experimental defects (31%) (P= NS). The new bone percentage was reduced from the peripheral zone toward the middle and the center of the defect (31%, 32% and 27%, respectively) as the distance from the peripheral pristine bone borders increased. The existing data support the complete degradation of CCG as space-maintaining scaffold for GBR procedures.
摘要：
天然珊瑚颗粒（NCP）是引导骨再生（GBR）手术的合适支架材料；它结合了支撑屏障膜的骨替代物的放置。由于日益增加的海洋污染和濒危珊瑚物种的申报（KYOTO1997），它们不再适合医疗行业。新型的国内珊瑚已在受控条件下生长，以生产栽培珊瑚接枝（CCG）材料。本研究旨在在体内实验性GBR程序中评估新的CCG。手术暴露了8只兔子的颅骨，制备直径8mm的圆形缺陷。一个缺损用CCG颗粒填充（实验组）；对侧缺损（对照组）由血凝块自发填充。用胶原膜覆盖缺损。8周后将动物安乐死。缺损的组织学观察显示，在实验和对照截骨术中，骨生长方式相似。在实验缺陷中，没有观察到珊瑚颗粒的痕迹。组织学分析显示，对于对照（50％）和实验缺陷（31％）（P=NS），原始区域（65-66％）的骨密度高于外围区域。新骨百分比从缺损的外围区域向中间和中心减少（31％，32%和27%，分别）随着与周围原始骨边界的距离增加。现有数据支持CCG作为GBR程序的空间维持支架的完全降解。
