Abstract：
Surveillance data collected in the period 2017-20 for Brucella spp. in wildlife of the Lombardy Region in northern Italy were used to describe the exposure of the wildlife species to Brucella spp. in wild boar (Sus scrofa), European brown hare (Lepus europaeus), fallow deer (Dama dama), red deer (Cervus elaphus), and roe deer (Capreolus capreolus). Among the tested species, wild boar (n=6,440) showed the highest percentage of seropositive samples (5.9%). Notably, wild boars of perifluvial area of the Po River showed higher percentages of positivity than those of the pre-Alpine district. In addition, during the hunting season in 2018, 95 organs (uterus or testes, spleen, and submandibular lymph nodes) from wild boar of the perifluvial area of the Po River were collected for bacteriological examination. Brucella suis was isolated in culture from 18.9% of tested lymph nodes. These serological and microbiological results highlight the presence of B. suis in wild boar and suggest the importance of wild boar as a reservoir for B. suis. Comparison of the spatial distribution of Brucella-seropositive wild boars with the location of backyard swine farms revealed a higher chance of contact between the two populations only in the areas where the lower percentage of seropositive samples was observed. Conversely, the high percentage of seropositive samples observed in the Po River area coupled with positive microbiological cultures suggest a greater risk of infection for the humans directly or indirectly involved in wild boar hunting activity. These results may serve as a basis to establish sound wildlife management and to adopt education campaigns aimed at reducing the risk of human infection in people involved in wild boar hunting related activities.
摘要：
2017-20年期间收集的布鲁氏菌属监测数据。在意大利北部伦巴第大区的野生动植物中，用于描述野生动植物物种对布鲁氏菌属的暴露。在野猪(Susscrofa)中，欧洲棕色野兔（Lepuseuropaeus），小鹿（DamaDama），马鹿(Cervuselaphus),和ro（Capreoluscapreolus）。在测试的物种中，野猪（n=6,440）的血清阳性样本百分比最高（5.9％）。值得注意的是,Po河沿岸地区的野猪比高山前地区的野猪表现出更高的阳性百分比。此外,在2018年的狩猎季节，95个器官(子宫或睾丸，脾,脾和颌下淋巴结）从Po河周围地区的野猪中收集进行细菌学检查。猪布鲁氏菌从18.9％的测试淋巴结中分离出培养物。这些血清学和微生物学结果强调了野猪中猪链球菌的存在，并表明了野猪作为猪链球菌储库的重要性。将布鲁氏菌血清阳性野猪的空间分布与后院猪场的位置进行比较，发现仅在观察到血清阳性样本百分比较低的地区，这两个种群之间的接触机会更高。相反，在波河地区观察到的高比例的血清阳性样本以及阳性的微生物培养物表明，直接或间接参与野猪狩猎活动的人类感染的风险更大。这些结果可以作为建立健全的野生动植物管理和采取旨在减少参与野猪狩猎相关活动的人感染人类的风险的教育运动的基础。
