Abstract：
OBJECTIVE: This study aims to assess ultrashort-TE magnetization transfer (UTE-MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.
METHODS: Thirty-six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE-MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0 w) and from water to the macromolecular pool (Rm0 m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0 w, Rm0 m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE-MT parameters to predict collagen degradation.
RESULTS: MMF, T2m, R1m, Rm0 m, and MTR decreased after digestion. MMF (r = -0.842, p < 0.001), MTR (r = -0.78, p < 0.001), and Rm0 m (r = -0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm0 m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.
CONCLUSIONS: This study highlighted the potential of UTE-MT parameters for evaluation of supraspinatus tendinopathy.
METHODS: Thirty-six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE-MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0 w) and from water to the macromolecular pool (Rm0 m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0 w, Rm0 m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE-MT parameters to predict collagen degradation.
RESULTS: MMF, T2m, R1m, Rm0 m, and MTR decreased after digestion. MMF (r = -0.842, p < 0.001), MTR (r = -0.78, p < 0.001), and Rm0 m (r = -0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm0 m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.
CONCLUSIONS: This study highlighted the potential of UTE-MT parameters for evaluation of supraspinatus tendinopathy.
摘要：
目的：本研究旨在使用肩袖肌腱病的体外模型评估胶原蛋白降解的超短TE磁化转移（UTE-MT）成像。
方法：将36例冈上肌腱标本分为3组，用600U胶原酶处理（第1组），150U胶原酶（第2组），和磷酸盐缓冲盐水(组3)。进行UTE-MT成像以评估大分子分数（MMF）的变化，大分子横向弛豫时间（T2m），水纵向弛豫速率常数(R1m)，从大分子到水池(Rm0w)和从水到大分子池(Rm0m)的磁化交换速率，基线和消化后的磁化转移比（MTR）及其组间差异。进行生化和组织学研究以确定胶原降解的程度。用MMF进行相关分析，T2m,R1m,Rm0w,Rm0米，和地铁，分别。进行单变量和多变量线性回归分析以评估UTE-MT参数的组合以预测胶原降解。
结果：MMF，T2m,R1m,Rm0米，消化后MTR下降。MMF（r=-0.842，p<0.001），MTR（r=-0.78，p<0.001），Rm0m(r=-0.662,p<0.001)与胶原降解呈显著负相关。消化前后MMF和Rm0m差异的线性回归模型解释了肌腱中胶原降解变异的68.9%。MMF和T2m消化后模型和MTR模型解释了胶原降解变化的54.2%和52.3%，分别。
结论：本研究强调了UTE-MT参数用于评价冈上肌腱病的潜力。
方法：将36例冈上肌腱标本分为3组，用600U胶原酶处理（第1组），150U胶原酶（第2组），和磷酸盐缓冲盐水(组3)。进行UTE-MT成像以评估大分子分数（MMF）的变化，大分子横向弛豫时间（T2m），水纵向弛豫速率常数(R1m)，从大分子到水池(Rm0w)和从水到大分子池(Rm0m)的磁化交换速率，基线和消化后的磁化转移比（MTR）及其组间差异。进行生化和组织学研究以确定胶原降解的程度。用MMF进行相关分析，T2m,R1m,Rm0w,Rm0米，和地铁，分别。进行单变量和多变量线性回归分析以评估UTE-MT参数的组合以预测胶原降解。
结果：MMF，T2m,R1m,Rm0米，消化后MTR下降。MMF（r=-0.842，p<0.001），MTR（r=-0.78，p<0.001），Rm0m(r=-0.662,p<0.001)与胶原降解呈显著负相关。消化前后MMF和Rm0m差异的线性回归模型解释了肌腱中胶原降解变异的68.9%。MMF和T2m消化后模型和MTR模型解释了胶原降解变化的54.2%和52.3%，分别。
结论：本研究强调了UTE-MT参数用于评价冈上肌腱病的潜力。
