PDF(Pubmed)
Abstract：
The endocrine pancreas is composed of clusters of cell groups called pancreatic islets. These cells are responsible for the synthesis and secretion of hormones crucial for glycemic homeostasis, such as insulin and glucagon. Therefore, these cells were the targets of many studies. One method to study and/or understand endocrine pancreatic physiology is the isolation of these islets and stimulation of hormone production using different concentrations of glucose, agonists, and/or antagonists of specific secretagogues and mimicking the stimulation of hormonal synthesis and secretion. Many researchers studied pancreatic physiology in murine models due to their ease of maintenance and rapid development. However, the isolation of pancreatic islets involves meticulous processes that may vary between rodent species. The present study describes a simple and effective technical protocol for isolating intact islets from mice and rats for use as a practical guide for researchers. The method involves digestion of the acinar parenchyma by intraductal collagenase. Isolated islets are suitable for in vitro endocrine secretion analyses, microscopy techniques, and biochemical analyses.
摘要：
内分泌胰腺由称为胰岛的细胞群的簇组成。这些细胞负责合成和分泌对血糖稳态至关重要的激素，如胰岛素和胰高血糖素。因此,这些细胞是许多研究的目标。研究和/或了解内分泌胰腺生理学的一种方法是分离这些胰岛并使用不同浓度的葡萄糖刺激激素产生。激动剂,和/或特定促分泌素的拮抗剂并模拟激素合成和分泌的刺激。许多研究人员在鼠模型中研究了胰腺生理学，因为它们易于维护和快速发展。然而,胰岛的分离涉及细致的过程，可能因啮齿动物物种而异。本研究描述了一种简单有效的技术方案，用于从小鼠和大鼠中分离完整的胰岛，作为研究人员的实用指南。该方法涉及通过导管内胶原酶消化腺泡实质。分离的胰岛适用于体外内分泌分泌分析，显微镜技术,和生化分析。
