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Abstract：
OBJECTIVE: To elucidate the epidemiological features of HIV-2 in Hunan Province, China, utilizing sequence analysis.
METHODS: Thirteen individuals diagnosed with HIV-2 infection in Hunan Province, China, from 2017 to 2023 were included in this study. Amplification of HIV-2 env and pol regions was conducted, followed by Sanger sequencing. Phylogenetic and molecular transmission network analyses were performed to delineate molecular features and transmission dynamics.
RESULTS: All 14 individuals contracted HIV-2 through heterosexual intercourse, comprising 7 males and 7 females, with a median age of 58 years. Among them, three couples (HN001 and HN013, HN010 and HN011, HN008 and HN009) were identified, along with commercial sexual activity engagement reported for subject HN004. Notably, subjects HN001, HN003, HN008, and HN010 engaged in commercial sexual activities at the same location as subject HN004. Phylogenetic analysis of the pol gene revealed close proximity of sequences from all subjects to reference sequences from Gambia (Sub-type A). Employing a genetic distance threshold of 1.5 %, eight out of the 14 subjects formed a molecular transmission network, with HN002 and HN004 identified as central nodes.
CONCLUSIONS: From 2017 to 2023, all HIV-2-infected individuals in Hunan Province, China, acquired the virus through identifiable routes, indicating transmission of similar HIV-2 strains among them.
METHODS: Thirteen individuals diagnosed with HIV-2 infection in Hunan Province, China, from 2017 to 2023 were included in this study. Amplification of HIV-2 env and pol regions was conducted, followed by Sanger sequencing. Phylogenetic and molecular transmission network analyses were performed to delineate molecular features and transmission dynamics.
RESULTS: All 14 individuals contracted HIV-2 through heterosexual intercourse, comprising 7 males and 7 females, with a median age of 58 years. Among them, three couples (HN001 and HN013, HN010 and HN011, HN008 and HN009) were identified, along with commercial sexual activity engagement reported for subject HN004. Notably, subjects HN001, HN003, HN008, and HN010 engaged in commercial sexual activities at the same location as subject HN004. Phylogenetic analysis of the pol gene revealed close proximity of sequences from all subjects to reference sequences from Gambia (Sub-type A). Employing a genetic distance threshold of 1.5 %, eight out of the 14 subjects formed a molecular transmission network, with HN002 and HN004 identified as central nodes.
CONCLUSIONS: From 2017 to 2023, all HIV-2-infected individuals in Hunan Province, China, acquired the virus through identifiable routes, indicating transmission of similar HIV-2 strains among them.
摘要：
目的:阐明湖南省HIV-2的流行病学特征,中国,利用序列分析。
方法：湖南省13例HIV-2感染者,中国,从2017年到2023年被纳入这项研究。进行了HIV-2env和pol区域的扩增，其次是Sanger测序。进行了系统发育和分子传输网络分析，以描绘分子特征和传输动力学。
结果：所有14个人通过异性性交感染HIV-2，包括7名男性和7名女性，平均年龄为58岁。其中,确定了三对夫妇（HN001和HN013，HN010和HN011，HN008和HN009），以及据报道受试者HN004的商业性活动参与。值得注意的是,受试者HN001、HN003、HN008和HN010在与受试者HN004相同的地点从事商业性活动。pol基因的系统发育分析表明，所有受试者的序列与冈比亚的参考序列（A型）非常接近。采用1.5%的遗传距离阈值，14个受试者中有8个形成了分子传输网络，将HN002和HN004标识为中心节点。
结论：2017年至2023年，湖南省所有HIV-2感染者，中国,通过可识别的途径获得病毒，表明它们之间有相似的HIV-2毒株传播。
方法：湖南省13例HIV-2感染者,中国,从2017年到2023年被纳入这项研究。进行了HIV-2env和pol区域的扩增，其次是Sanger测序。进行了系统发育和分子传输网络分析，以描绘分子特征和传输动力学。
结果：所有14个人通过异性性交感染HIV-2，包括7名男性和7名女性，平均年龄为58岁。其中,确定了三对夫妇（HN001和HN013，HN010和HN011，HN008和HN009），以及据报道受试者HN004的商业性活动参与。值得注意的是,受试者HN001、HN003、HN008和HN010在与受试者HN004相同的地点从事商业性活动。pol基因的系统发育分析表明，所有受试者的序列与冈比亚的参考序列（A型）非常接近。采用1.5%的遗传距离阈值，14个受试者中有8个形成了分子传输网络，将HN002和HN004标识为中心节点。
结论：2017年至2023年，湖南省所有HIV-2感染者，中国,通过可识别的途径获得病毒，表明它们之间有相似的HIV-2毒株传播。
