Abstract：
The family Sarcocystidae includes several intracellular coccidial parasites such as Toxoplasma gondii, Neospora caninum, Sarcocystis spp. and Hammondia spp. with heteroxenous life cycles involving different parasitic stages (oocysts/sporocysts, tachyzoites and bradyzoites in tissue cysts). The aim of this work was to evaluate monoclonal antibodies (MAb) (anti NcSAG1, anti NcSAG4 and anti TgCC2) and/or polyclonal antibodies (PAb) (anti NcSAG4 and anti TgBAG1) to label specific immunodominant antigens in different parasitic stages of N. caninum (oocyst, bradyzoite and tachyzoite), T. gondii (oocyst, cyst and tachyzoite), H. heydorni (oocyst), S. cruzi (cyst and bradyzoite) and S. falcatula (sporocyst). It was observed that the MAb directed against NcSAG1 reacted exclusively with N. caninum tachyzoites. In contrast, the MAb directed against NcSAG4 did not react with any of the parasites tested at any stage. The MAb directed against NcSAG4 reacted with both N. caninum and T. gondii tachyzoites, T. gondii tissue cysts and S. cruzi tissue cysts and bradyzoites. As expected, the MAb directed against the T. gondii tissue cyst wall antigen TgCC2 reacted with T. gondii tissue cysts, N. caninum bradyzoites, but also with T. gondii and H. heydorni oocysts and S. falcatula sporocysts. Finally, the PAb directed against the T. gondii bradyzoite proteinTgBAG1 reacted with T. gondii tissue cysts, N. caninum bradyzoites, and also with S. cruzi tissue cysts and bradyzoites. These data reveal a wide range of cross-reactions between different species of protozoa and between different developmental stages, which should be taken into account in the design and evaluation of diagnostic tests, as well as in the assessment of vaccination and challenge studies.
摘要：
该家族包括几种细胞内球虫寄生虫，例如弓形虫，新孢子虫,结节虫。和Hammondiaspp.具有涉及不同寄生阶段的异氧生命周期(卵囊/孢子囊，组织囊肿中的速殖子和缓生子）。这项工作的目的是评估单克隆抗体（MAb）（抗NcSAG1，抗NcSAG4和抗TgCC2）和/或多克隆抗体（PAb）（抗NcSAG4和抗TgBAG1），以标记不同寄生阶段的特异性免疫显性抗原。犬（卵囊，缓子和速殖子)，T.gondii（卵囊，囊肿和速殖子)，H.heydorni（卵囊），S.Cruzi（囊肿和缓生子）和S.falcatula（孢子囊）。观察到，针对NcSAG1的MAb仅与犬速殖子反应。相比之下,针对NcSAG4的MAb在任何阶段都不与任何测试的寄生虫反应。针对NcSAG4的MAb与犬奈瑟菌和弓形虫速殖子反应，弓形虫组织囊肿和克鲁子组织囊肿和缓生子。不出所料,针对弓形虫组织囊壁抗原TgCC2的MAb与弓形虫组织囊肿反应，N.犬齿缓子，还有弓形虫和H.heydorni卵囊和S.falcatula孢子囊。最后,针对弓形虫缓生蛋白TgBAG1的PAb与弓形虫组织囊肿反应，N.犬齿缓子，还有克氏链球菌组织囊肿和缓生。这些数据揭示了不同物种原生动物之间以及不同发育阶段之间的广泛交叉反应，在设计和评估诊断测试时应考虑到这一点，以及在疫苗接种和挑战研究的评估中。
