Abstract：
Selenium-rich tea polysaccharides (Se-TPS) were extracted via high hydrostatic pressure technology with a pressure of 400 MPa (200-500 MPa) for 10 min (3-20 min) at a material-to-solvent ratio of 1:40 (1:20-1:50). Subsequently, Se-TPS1-4 were isolated and purified, with Se-TPS3-4 as the main components. A spectral analysis proved that Se, which has antioxidant activity, existed. An in vitro study found that among Se-TPS, Se-TPS3-4 attenuated the release of β-hexosaminidase, histamine, and interleukin (IL)-4. Furthermore, in vivo experiments revealed that treatment with Se-TPS downregulated IL-4 levels and upregulated TGF-β and interferon-γ levels to improve imbalanced Th1/Th2 immunity in tropomyosin-sensitized mice. Moreover, Se-TPS promoted Lactobacillus and norank_f_Muribaculaceaek growth and upregulated metabolites such as genipin and coniferyl alcohol. Overall, these results showed the strong anti-allergy potential of Se-TPS by regulating mast cell-mediated allergic inflammatory responses and microbiota regulation, highlighting the potential of Se-TPS as a novel therapeutic agent to regulate allergy-associated metabolic disorders.
摘要：
通过高静水压力技术以400MPa（200-500MPa）的压力在10分钟（3-20分钟）的材料与溶剂比为1提取富硒茶多糖（Se-TPS）：40（1：20-1：50）。随后，Se-TPS1-4被分离和纯化，以Se-TPS3-4为主要成分。光谱分析证明Se,具有抗氧化活性，存在。一项体外研究发现，在Se-TPS中，Se-TPS3-4减弱β-己糖胺酶的释放，组胺,和白细胞介素（IL）-4。此外，体内实验表明，Se-TPS治疗下调IL-4水平，上调TGF-β和干扰素-γ水平，以改善原肌球蛋白致敏小鼠Th1/Th2免疫不平衡。此外，Se-TPS促进乳杆菌和Norank_f_Muribaculaceaek的生长，并上调代谢产物如京尼平和针叶醇。总的来说,这些结果表明，Se-TPS通过调节肥大细胞介导的过敏性炎症反应和微生物群调节具有很强的抗过敏潜力，强调Se-TPS作为调节过敏相关代谢紊乱的新型治疗剂的潜力。
