Abstract：
The surge in domestic cat adoption across India, particularly the rising preference for high-pedigree cats, coupled with environmental factors, has resulted in increased incidence of dermatophytosis among feline companions. Despite this growing concern, there is a noticeable scarcity of studies in India delving into the etiological factors contributing to dermatophytosis in cats. This disease is a threat to animal health and carries public health significance, given that cats are recognized reservoir hosts for Microsporum canis, a common dermatophyte affecting humans and animals. This study endeavours to identify the dermatophytes affecting cats and establish a standardized therapeutic regimen while accounting for the local stigma surrounding the regular bathing of cats. The study involved the examination of 82 cats presenting dermatological lesions, when subjected to cultural examination in dermatophyte test medium revealed 36 afflicted with dermatophytes. Isolates were presumptively identified by staining using lactophenol cotton blue, Chicago sky blue 6B, and Calcofluor white stains. Molecular-level identification of the isolates was confirmed through PCR-RFLP, amplifying the Internal Transcribed Spacer Sequence of 16 s rDNA, followed by restriction digestion using the Mva1 enzyme. Among the thirty-six isolates, 29 were identified as M. canis, while the remaining 7 were M. gypseum. The cases were categorized into five groups and treated with Lime Sulphur dip, 4 % chlorhexidine shampoo, a shampoo containing 2 % miconazole and 4 % chlorhexidine, oral itraconazole alone, and a combination of oral itraconazole with lime-Sulphur dip. Statistical analysis revealed that the response was notably swifter with lime Sulphur dip when considering only topical therapy. Moreover, the mycological cure was most expeditious when combining Lime Sulphur dip with oral itraconazole. These findings underscore the pivotal role of topical biocides in feline dermatophytosis treatment, potentially reducing the reliance on specific antifungals and thereby contributing to the mitigation of antimicrobial resistance emergence.
摘要：
印度家猫收养的激增，特别是对高血统猫的偏好上升，加上环境因素，导致猫科动物中皮肤癣菌病的发病率增加。尽管人们越来越担心,在印度，研究猫皮肤癣菌病的病因的研究明显缺乏。这种疾病对动物健康构成威胁，具有公共卫生意义，鉴于猫是犬小孢子菌的公认宿主，一种影响人类和动物的常见皮肤癣菌。这项研究努力确定影响猫的皮肤癣菌，并建立标准化的治疗方案，同时考虑到猫定期洗澡周围的局部污名。该研究包括对82只出现皮肤病的猫的检查，当在皮肤癣菌测试培养基中进行培养检查时，发现36种皮肤癣菌。通过使用乳酚棉蓝染色来推定鉴定分离物，芝加哥天蓝6B，和Calcofluor白色污渍。通过PCR-RFLP确认了分离株的分子水平鉴定，扩增16srDNA的内部转录间隔序列，然后使用Mva1酶进行限制性消化。在36个分离株中，29人被鉴定为M.canis，而剩下的7个是石膏。将病例分为五组，用石灰硫浸治疗，4%洗必泰洗发水，含有2%咪康唑和4%氯己定的洗发水，单独口服伊曲康唑，和口服伊曲康唑与石灰-硫磺浸渍的组合。统计分析显示，当仅考虑局部治疗时，石灰硫浸液的反应明显更快。此外，真菌学治疗是最迅速的，当石灰硫浸与口服伊曲康唑。这些发现强调了局部杀菌剂在猫科动物皮肤癣菌病治疗中的关键作用。有可能减少对特定抗真菌药物的依赖，从而有助于缓解抗菌素耐药性的出现。
