Abstract：
It is challenging to prepare sample pretreatment materials with simple use, strong selectivity and satisfactory enrichment performance. In this study, the antibody (3D4) that can specifically recognize zearalenone (ZEN) and its metabolites was immobilized on the surface of gold-coated magnetic Fe3O4 nanoparticles (GMN) by streptavidin (SA)-biotin interaction using GMN as the substrate and our designed four-arm PEG derivative (HS-4ARMPEG10K-(CM)3) as the linker. The immunomagnetic nanoparticles (GMN-4ARMPEG10K-SA-3D4) prepared by this strategy can achieve rapid enrichment (only 5 min) of analytes directly in the matrix, and higher enrichment capacity compared with the previous immunomagnetic particles. The sensitive and accurate analysis of ZEN and its metabolites can be achieved coupled with HPLC-MS/MS. The LODs and LOQs were 0.02-0.05 μg/kg and 0.05-0.10 μg/kg, respectively. The recoveries were 84.13%-112.67%, and the RSDs were 1.09%-9.39%. The method can provide a powerful tool for highly sensitive and rapid monitoring of mycotoxins in complex matrices due to its\' strong selectivity and resistance to matrix interference.
摘要：
用简单的方法制备样品预处理材料是具有挑战性的，强的选择性和令人满意的富集性能。在这项研究中,以GMN为底物，我们设计的四臂PEG衍生物（HS-4ARMPEG10K-（CM）3）作为接头，通过链霉亲和素（SA）-生物素相互作用，将能够特异性识别玉米赤霉烯酮（ZEN）及其代谢物的抗体（3D4）固定在金包被的磁性Fe3O4纳米颗粒（GMN）的表面。通过该策略制备的免疫磁性纳米颗粒（GMN-4ARMPEG10K-SA-3D4）可以直接在基质中实现分析物的快速富集（仅5分钟），与以前的免疫磁性颗粒相比，具有更高的富集能力。HPLC-MS/MS可实现对ZEN及其代谢物的灵敏、准确的分析。LOD和LOQs分别为0.02-0.05μg/kg和0.05-0.10μg/kg,分别。加标回收率为84.13%-112.67%,RSD为1.09%-9.39%。该方法具有很强的选择性和抗基质干扰能力，可为复杂基质中真菌毒素的高灵敏度和快速监测提供有力工具。
