Abstract：
The most significant and sensitive antigen protein that causes diarrhea in weaned pigs is soybean 7S globulin. Therefore, identifying the primary target for minimizing intestinal damage brought on by soybean 7S globulin is crucial. MicroRNA (miRNA) is closely related to intestinal epithelium\'s homeostasis and integrity. However, the change of miRNAs\' expression and the function of miRNAs in Soybean 7S globulin injured-IPEC-J2 cells are still unclear. In this study, the miRNAs\' expression profile in soybean 7S globulin-treated IPEC-J2 cells was investigated. Fifteen miRNAs were expressed differently. The differentially expressed miRNA target genes are mainly concentrated in signal release, cell connectivity, transcriptional inhibition, and Hedgehog signaling pathway. Notably, we noticed that the most significantly decreased miRNA was ssc-miR-221-5p after soybean 7S globulin treatment. Therefore, we conducted a preliminary study on the mechanisms of ssc-miR-221-5p in soybean 7S globulin-injured IPEC-J2 cells. Our research indicated that ssc-miR-221-5p may inhibit ROS production to alleviate soybean 7S globulin-induced apoptosis and inflammation in IPEC-J2 cells, thus protecting the cellular mechanical barrier, increasing cell proliferation, and improving cell viability. This study provides a theoretical basis for the prevention and control of diarrhea of weaned piglets.
摘要：
引起断奶仔猪腹泻的最显著和最敏感的抗原蛋白是大豆7S球蛋白。因此,确定最大程度减少大豆7S球蛋白对肠道损伤的主要目标至关重要。微小RNA(miRNA)与肠上皮的稳态和完整性密切相关。然而,大豆7S球蛋白损伤的IPEC-J2细胞中miRNAs的表达变化和miRNAs的功能尚不清楚。在这项研究中,研究了大豆7S球蛋白处理的IPEC-J2细胞中miRNA的表达谱。15种miRNA表达不同。miRNA靶基因的差异表达主要集中在信号释放,细胞连通性，转录抑制，和Hedgehog信号通路。值得注意的是,我们注意到，大豆7S球蛋白处理后，miRNA下降最显著的是ssc-miR-221-5p。因此,我们对ssc-miR-221-5p在大豆7S球蛋白损伤IPEC-J2细胞中的作用机制进行了初步研究。我们的研究表明ssc-miR-221-5p可能通过抑制ROS的产生来减轻大豆7S球蛋白诱导的IPEC-J2细胞凋亡和炎症反应,从而保护细胞机械屏障，增加细胞增殖，提高细胞活力。本研究为断奶仔猪腹泻的防治提供了理论依据。
