关键词: Turicibacter gut microbiota new novel

Mesh : Animals RNA, Ribosomal, 16S / genetics Feces / microbiology Phylogeny Mice Base Composition DNA, Bacterial / genetics Bacterial Typing Techniques Sequence Analysis, DNA Heart Failure / microbiology Disease Models, Animal Genome, Bacterial Male Fatty Acids

来  源:   DOI:10.1099/ijsem.0.006379   PDF(Pubmed)

Abstract:
Strain TC023T, a Gram-positive, long, rod-shaped, spore-forming anaerobe, was isolated from the faeces of a heart failure mouse model. The strain formed greyish-white coloured colonies with a convex elevation on brain-heart infusion medium supplemented with 0.1 % sodium taurocholate, incubated at 37 °C for 2 days. Taxonomic analysis based on the 16S rRNA gene sequence showed that TC023T belonged to the genus Turicibacter, and was closely related to Turicibacter bilis MMM721T (97.6 %) and Turicibacter sanguinis MOL361T (97.4 %). The whole genome of the strain has a G+C content of 37.3 mol%. The average nucleotide identity and genome-to-genome distance between TC023T and Turicibacter bilis MMM721T were 77.6 % and 24.3 %, respectively, and those with Turicibacter sanguinis MOL361T were 75.4 % and 24.3 %, respectively. These genotypic, phenotypic, and biochemical analyses indicated that the isolate represents a novel species in the genus Turicibacter, and the name Turicibacter faecis sp. nov. is proposed. The type strain is TC023T (RIMD 2002001T=TSD 372T).
摘要:
菌株TC023T,革兰氏阳性,长,杆状,孢子形成厌氧菌,从心力衰竭小鼠模型的粪便中分离。该菌株在补充有0.1%牛磺胆酸钠的脑心输注培养基上形成灰白色的菌落,并有凸起的高度,在37°C孵育2天。基于16SrRNA基因序列的分类学分析表明,TC023T属于Turicibacter属,与胆汁TuricibacterMMM721T(97.6%)和血液TuricibacterMOL361T(97.4%)密切相关。该菌株的全基因组具有37.3mol%的G+C含量。TC023T和结核分枝杆菌MMM721T的平均核苷酸同一性和基因组间距离分别为77.6%和24.3%,分别,血TuricibacterMOL361T分别为75.4%和24.3%,分别。这些基因型,表型,生化分析表明,该分离株代表了Turicibacter属中的一个新物种,和名称Turicibacterfaecissp。11月。是提议的。菌株类型为TC023T(RIMD2002001T=TSD372T)。
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