Abstract：
In recent years, blueberry root rot has been caused mainly by Fusarium commune, and there is an urgent need for a green and efficient method to control this disease. To date, research on Schizophyllum commune has focused on antioxidant mechanisms, reactive dye degradation, etc., but the mechanism underlying the inhibition of pathogenic microorganisms is still unclear. Here, the control effects of S. commune on F. commune and blueberry root rot were studied using adversarial culture, tissue culture, and greenhouse pot experiments. The results showed that S. commune can dissolve insoluble phosphorus and secrete various extracellular hydrolases. The results of hyphal confrontation and fermentation broth antagonism experiments showed that S. commune had a significant inhibitory effect on F. commune, with inhibition rates of 70.30% and 22.86%, respectively. Microscopy results showed distortion of F. commune hyphae, indicating that S. commune is strongly parasitic. S. commune had a significant growth-promoting effect on blueberry tissue-cultured seedlings. After inoculation with S. commune, inoculation with the pathogenic fungus, or inoculation at a later time, the strain significantly reduced the root rot disease index in the potted blueberry seedlings, with relative control effects of 79.14% and 62.57%, respectively. In addition, S. commune G18 significantly increased the antioxidant enzyme contents in the aboveground and underground parts of potted blueberry seedlings. We can conclude that S. commune is a potential biocontrol agent that can be used to effectively control blueberry root rot caused by F. commune in the field.
摘要：
近年来,蓝莓根腐病主要由镰刀菌引起，迫切需要一种绿色有效的方法来控制这种疾病。迄今为止,对裂殖菌的研究集中在抗氧化机制上，活性染料降解，等。,但是抑制病原微生物的潜在机制仍不清楚。这里,利用对抗性培养研究了S.commune对F.commune和蓝莓根腐病的防治效果，组织培养,和温室盆栽实验。结果表明，S.commune可以溶解不溶性磷并分泌各种细胞外水解酶。菌丝对抗和发酵液拮抗实验结果表明，S.commune对F.commune有明显的抑制作用，抑制率分别为70.30％和22.86％，分别。显微镜检查结果显示F.commune菌丝变形，表明S公社是强烈寄生的。美国公社对蓝莓组培苗有显著的促生长作用。接种S公社后，接种致病真菌，或在稍后的时间接种，该菌株显著降低了盆栽蓝莓幼苗的根腐病指数，相对防治效果分别为79.14%和62.57%，分别。此外,S.communeG18显着增加了盆栽蓝莓幼苗的地上和地下部分的抗氧化酶含量。我们可以得出结论，S.commune是一种潜在的生物防治剂，可用于有效防治田间F.commune引起的蓝莓根腐病。
