PDF(Pubmed)
Abstract：
Macropodid alphaherpesvirus 2 (MaAHV2) is best described in macropods and has been implicated in outbreaks among captive marsupial populations in Australia. Natural disease caused by herpesviruses has not been reported previously in opossum species, to our knowledge. One Virginia opossum (Didelphis virginiana) and 1 water opossum (Chironectes minimus) were submitted for postmortem examination from a zoo that housed 6 opossums, all of which died within several weeks. Red kangaroos (Macropus rufus) and red-necked wallabies (Macropus rufogriseus) were also present at the facility. Liver samples from both opossums were submitted for transmission electron microscopy and whole-genome sequencing. Microscopically, both opossums had multifocal necrosis in the liver and lung, with intranuclear inclusion bodies within hepatocytes and pneumocytes. Another significant finding in the Virginia opossum was sepsis, with isolation of Streptococcus didelphis from various organs. Ultrastructural analysis of formalin-fixed liver tissue identified herpesviral replication complexes in both opossums; negative-stain electron microscopy of unfixed liver tissue repeatedly yielded a negative result. The herpesvirus had >99% nucleotide identity with MaAHV2. These 2 cases indicate that both opossum species are susceptible to MaAHV2 infection, and the outbreak has implications for mixed-species facilities that house macropods.
摘要：
巨足α疱疹病毒2（MaAHV2）在巨足类动物中的描述最好，并且与澳大利亚圈养有袋种群的爆发有关。由疱疹病毒引起的自然疾病以前在负鼠物种中没有报道，我们的知识。一个弗吉尼亚负鼠（Didelphisvirginiana）和1个负鼠（Chironectesminimus）从一个装有6个负鼠的动物园提交给验尸，所有这些都在几周内死亡。该设施还存在红袋鼠（Macropusrufus）和红颈小袋鼠（Macropusrufogriseus）。将来自两个负鼠的肝脏样品进行透射电子显微镜和全基因组测序。微观上,两个负鼠在肝脏和肺部都有多灶性坏死，肝细胞和肺细胞内有核内包涵体。弗吉尼亚负鼠的另一个重要发现是败血症，从各种器官中分离双链球菌。福尔马林固定的肝组织的超微结构分析确定了两个负鼠中的疱疹病毒复制复合物；未固定的肝组织的阴性染色电子显微镜反复产生阴性结果。疱疹病毒与MaAHV2具有>99%的核苷酸同一性。这2例病例表明两种负鼠都容易感染MaAHV2，疫情对容纳大型足类动物的混合物种设施产生了影响。
