PDF(Pubmed)
Abstract：
Cecal microbiota has emerged as a prominent intervention target for improving the production and welfare of poultry. This is essential for the overall health and performance of broiler chickens. The current study focused on investigating the effect of cecal microbiota transplantation (CMT) from healthy donor chickens on the growth performance, immunity, and microbial composition of newly hatched chicks and evaluated the effect of sample storage on the microbial diversity of the cecal samples. A healthy \"Wannan Yellow Chicken line\" was selected as the donor, and 180 1-d-old chicks from the same line were used as recipients for a 60-d feed trial. The chicks were randomly allocated to three groups (60 birds per group) with three replicates in each group. The three treatment groups were CMT-0 (control, normal saline solution), CMT-I (1:12 cecal content, normal saline supplemented with 10% glycerol), and CMT-II (1:6 cecal content, normal saline supplemented with 10% glycerol). The results of weight gain and absolute organ weight showed significant improvements in the CMT-II group compared with the CMT-0 group. Serum IgG level was significantly improved (P < 0.05) in CMT-I compared with that in the CMT-0. However, IL-6 levels increased in CMT-I and then significantly decreased in CMT-II. The cecal microbial diversity of CMT treatment was compared between two groups, fresh samples (FS) and stored samples at-80 °C (SS). The results showed that beneficial taxa, such as Firmicutes and Verrucomicrobiota, were substantially more abundant in both CMT-I and CMT-II than in CMT-0 in both FS and SS. Microbial function analysis at levels 1, 2, and 3 showed improved metabolism, genetic information processing, cellular processes, environmental information processing, and organismal systems in CMT-I and CMT-II for both FS and SS groups. However, the SS group showed decreased microbial diversity and function. To conclude, cecal microbiota transplantation is a promising strategy for enhancing the productivity and health of broiler chickens.
The cecal microbiota refers to a diverse community of microorganisms that play a crucial role in digestion, nutrient absorption, and overall gut health, influencing the well-being and performance of the host bird. In this study, we aimed to improve the health and growth of broiler chickens by exploring a unique approach called cecal microbiota transplantation. A thorough investigation was conducted by transplanting the microbiota from healthy Wannan Yellow Chicken line donors into newly hatched chicks in a 60-d feeding trial. After dividing the chicks into three groups, each receiving different treatments, we found significant enhancements in WG and organ health in the groups that received cecal microbiota transplants. The results also showed improvements in Serum IgG levels in the treatment groups. Furthermore, the analysis of microbial diversity indicated that beneficial microorganisms were more abundant in the treated groups, suggesting a positive effect on chicken digestive health. To summarize, our findings suggest that transferring healthy gut microorganisms from mature parent chickens to young chicks can lead to improved growth, immune system function, microbial diversity, and overall health. This approach is a promising strategy for enhancing the productivity and well-being of broiler chickens.
The cecal microbiota refers to a diverse community of microorganisms that play a crucial role in digestion, nutrient absorption, and overall gut health, influencing the well-being and performance of the host bird. In this study, we aimed to improve the health and growth of broiler chickens by exploring a unique approach called cecal microbiota transplantation. A thorough investigation was conducted by transplanting the microbiota from healthy Wannan Yellow Chicken line donors into newly hatched chicks in a 60-d feeding trial. After dividing the chicks into three groups, each receiving different treatments, we found significant enhancements in WG and organ health in the groups that received cecal microbiota transplants. The results also showed improvements in Serum IgG levels in the treatment groups. Furthermore, the analysis of microbial diversity indicated that beneficial microorganisms were more abundant in the treated groups, suggesting a positive effect on chicken digestive health. To summarize, our findings suggest that transferring healthy gut microorganisms from mature parent chickens to young chicks can lead to improved growth, immune system function, microbial diversity, and overall health. This approach is a promising strategy for enhancing the productivity and well-being of broiler chickens.
摘要：
盲肠微生物群已成为改善家禽生产和福利的重要干预目标。这对于肉鸡的整体健康和性能至关重要。目前的研究集中在调查盲肠微生物移植（CMT）从健康的供体鸡的生长性能的影响，豁免权,和新孵化雏鸡的微生物组成，并评估了样品储存对盲肠样品微生物多样性的影响。一个健康的"皖南黄鸡系"被选为捐献者,来自同一品系的180只1日龄雏鸡被用作60天饲料试验的接受者。将小鸡随机分为三组(每组60只),每组重复三次。三个治疗组均为CMT-0(对照组,生理盐水溶液)，CMT-I（1:12盲肠内容物，补充有10％甘油的生理盐水），和CMT-II(1:6盲肠含量，补充有10%甘油的生理盐水)。与CMT-0组相比，CMT-II组的体重增加和绝对器官重量的结果显示显着改善。与CMT-0相比，CMT-I的血清IgG水平显着提高（p<0.05）。然而,IL-6水平在CMT-I中升高,然后在CMT-II中显著降低。比较两组CMT治疗的盲肠微生物多样性,新鲜样品(FS)和在-80°C(SS)下储存的样品。结果表明，有益分类单元，例如Firmicutes和Verrucomicroota，在FS和SS中，CMT-I和CMT-II的含量均比CMT-0的含量高。水平1、2和3的微生物功能分析显示代谢改善，遗传信息处理，细胞过程，环境信息处理,以及FS和SS组的CMT-I和CMT-II中的有机系统。然而,SS组微生物多样性和功能下降。最后，盲肠微生物移植是提高肉鸡生产力和健康的一个有前途的策略。
