Abstract：
Cryptochromes (CRYs) act as blue light photoreceptors to regulate various plant physiological processes including photomorphogenesis and repair of DNA double strand breaks (DSBs). ADA2b is a conserved transcription co-activator that is involved in multiple plant developmental processes. It is known that ADA2b interacts with CRYs to mediate blue light-promoted DSBs repair. Whether ADA2b may participate in CRYs-mediated photomorphogenesis is unknown. Here we show that ADA2b acts to inhibit hypocotyl elongation and hypocotyl cell elongation in blue light. We found that the SWIRM domain-containing C-terminus mediates the blue light-dependent interaction of ADA2b with CRYs in blue light. Moreover, ADA2b and CRYs act to co-regulate the expression of hypocotyl elongation-related genes in blue light. Based on previous studies and these results, we propose that ADA2b plays dual functions in blue light-mediated DNA damage repair and photomorphogenesis.
摘要：
隐色素（CRY）充当蓝光光感受器，以调节各种植物生理过程，包括光形态发生和DNA双链断裂（DSB）的修复。ADA2b是一种保守的转录共激活因子，参与多种植物发育过程。已知ADA2b与CRY相互作用以介导蓝光促进的DSB修复。ADA2b是否可能参与CRYs介导的光形态发生尚不清楚。在这里，我们显示ADA2b在蓝光下抑制下胚轴伸长和下胚轴细胞伸长。我们发现，含有SWIRM结构域的C末端介导了蓝光下ADA2b与CRY的蓝光依赖性相互作用。此外，ADA2b和CRYs共同调节蓝光下胚轴伸长相关基因的表达。根据以前的研究和这些结果，我们认为ADA2b在蓝光介导的DNA损伤修复和光形态发生中起着双重作用。
