PDF(Pubmed)
Abstract：
Tracking small extracellular vesicles (sEVs), such as exosomes, requires staining them with dyes that penetrate their lipid bilayer, a process that leaves excess dye that needs to be mopped up to achieve high specificity. Current methods to remove superfluous dye have limitations, among them that they are time-intensive, carry the risk of losing sample and can require specialized equipment and materials. Here we present a fast, easy-to-use, and cost-free protocol for cleaning excess dye from stained sEV samples by adding their parental cells to the mixture to absorb the extra dye much like sponges do. Since sEVs are considered a next-generation drug delivery system, we further show the success of our approach at removing excess chemotherapeutic drug, daunorubicin, from the sEV solution.
摘要：
追踪小细胞外囊泡（sEV），比如外泌体，需要用穿透它们的脂质双层的染料染色，留下多余染料的过程，需要清除以实现高特异性。目前去除多余染料的方法有局限性，其中，它们是时间密集型的，有丢失样品的风险，可能需要专门的设备和材料。在这里，我们提出了一个快速，易于使用，以及通过将其亲代细胞添加到混合物中以像海绵一样吸收额外的染料来从染色的sEV样品中清洁过量染料的无成本方案。由于sEV被认为是下一代药物递送系统，我们进一步展示了我们的方法在去除过量化疗药物方面的成功，柔红霉素,从sEV解决方案。
