PDF(Pubmed)
Abstract：
UNASSIGNED: Gelatin hydrogel nonwoven fabrics (Genocel) are three-dimensional gelatin scaffolds that provide cells with space for proliferation, migration, and differentiation. They are expected to be an effective wound healing modality to treat intractable wounds, such as diabetic foot ulcers, because they enhance early neovascularization when used as a skin substitute. In this study, we explored the healing process of Genocel applied to skin defects in diabetic mice and compared it with that of a conventional skin substitute, Pelnac.
UNASSIGNED: Genocel and Pelnac sheets were used to treat skin defects on the backs of diabetic mice. On days 7 and 14, the remaining wound area was evaluated and specimens were harvested for HE, Azan, anti-CD31, CD68, and CD163 staining to assess neoepithelialization, granulation tissue formation, capillary formation, and macrophage infiltration.
UNASSIGNED: Wounds treated with Genocel showed a wound healing process comparable to that of wounds treated with Pelnac. No significant differences were observed in the remaining wound area, neoepithelial length, granulation formation, number of pan-macrophages, or M2 ratio on days 7 and 14. The only significant difference was the number of induced M2 macrophages, which was higher in Pelnac group than in the Genocel group on day 7 (p < 0.05).
UNASSIGNED: Genocel showed similar healing effects in diabetic wounds as Pelnac and is considered an effective wound management modality for diabetic ulcers.
UNASSIGNED: Genocel and Pelnac sheets were used to treat skin defects on the backs of diabetic mice. On days 7 and 14, the remaining wound area was evaluated and specimens were harvested for HE, Azan, anti-CD31, CD68, and CD163 staining to assess neoepithelialization, granulation tissue formation, capillary formation, and macrophage infiltration.
UNASSIGNED: Wounds treated with Genocel showed a wound healing process comparable to that of wounds treated with Pelnac. No significant differences were observed in the remaining wound area, neoepithelial length, granulation formation, number of pan-macrophages, or M2 ratio on days 7 and 14. The only significant difference was the number of induced M2 macrophages, which was higher in Pelnac group than in the Genocel group on day 7 (p < 0.05).
UNASSIGNED: Genocel showed similar healing effects in diabetic wounds as Pelnac and is considered an effective wound management modality for diabetic ulcers.
摘要：
■明胶水凝胶无纺布（Genocel）是三维明胶支架，为细胞提供增殖空间，迁移,和差异化。它们有望成为治疗顽固性伤口的有效伤口愈合方式，比如糖尿病足溃疡，因为当用作皮肤替代品时，它们会增强早期新生血管形成。在这项研究中,我们探索了Genocel应用于糖尿病小鼠皮肤缺损的愈合过程，并将其与传统皮肤替代品进行了比较，Pelnac.
■使用Genocel和Pelnac片治疗糖尿病小鼠背部的皮肤缺损。在第7天和第14天，评估剩余的伤口面积，并收获标本进行HE，阿赞,抗CD31，CD68和CD163染色以评估新上皮形成，肉芽组织形成，毛细管形成，和巨噬细胞浸润。
■用Genocel治疗的伤口显示出与用Pelnac治疗的伤口相当的伤口愈合过程。在剩余的伤口区域没有观察到显著差异,上皮内瘤长度，造粒形成，泛巨噬细胞的数量，或第7天和第14天的M2比率。唯一的显著差异是诱导的M2巨噬细胞的数量，在第7天，Pelnac组高于Genocel组（p<0.05）。
■Genocel在糖尿病伤口中显示出与Pelnac相似的愈合效果，被认为是糖尿病溃疡的有效伤口管理方式。
■使用Genocel和Pelnac片治疗糖尿病小鼠背部的皮肤缺损。在第7天和第14天，评估剩余的伤口面积，并收获标本进行HE，阿赞,抗CD31，CD68和CD163染色以评估新上皮形成，肉芽组织形成，毛细管形成，和巨噬细胞浸润。
■用Genocel治疗的伤口显示出与用Pelnac治疗的伤口相当的伤口愈合过程。在剩余的伤口区域没有观察到显著差异,上皮内瘤长度，造粒形成，泛巨噬细胞的数量，或第7天和第14天的M2比率。唯一的显著差异是诱导的M2巨噬细胞的数量，在第7天，Pelnac组高于Genocel组（p<0.05）。
■Genocel在糖尿病伤口中显示出与Pelnac相似的愈合效果，被认为是糖尿病溃疡的有效伤口管理方式。
