PDF(Pubmed)
Abstract：
OBJECTIVE: To explore how the circular non-coding RNA circ_0072088 influences the progression of breast carcinoma (BC) by affecting cell behavior.
METHODS: We measured the levels of circ_0072088, microRNA-607 (miR-607), and ring finger protein 2 (RNF2) mRNA levels in BC tissues and cell lines using quantitative real-time PCR. We also conducted cell counting kit-8 (CCK-8), BrdU incorporation, and flow cytometry assays to assess cell viability, cell cycle, and apoptosis, respectively.
RESULTS: We observed increased levels of circ_0072088 and RNF2, and decreased levels of miR-607 in BC tissues. Overexpressing circ_0072088 promoted BC cell proliferation and cell cycle progression while inhibiting apoptosis. Conversely, silencing circ_0072088 had the opposite effects. Our data suggest that circ_0072088 directly targets and downregulates miR-607, which in turn upregulates RNF2, a target of miR-607. Moreover, miR-607 overexpression could mitigate the pro-proliferative and anti-apoptotic effects of circ_0072088 on BC cells.
CONCLUSIONS: Circ_0072088 drives BC progression by downregulating miR-607 and upregulating RNF2, thereby promoting cell proliferation and cycle progression while reducing apoptosis.
METHODS: We measured the levels of circ_0072088, microRNA-607 (miR-607), and ring finger protein 2 (RNF2) mRNA levels in BC tissues and cell lines using quantitative real-time PCR. We also conducted cell counting kit-8 (CCK-8), BrdU incorporation, and flow cytometry assays to assess cell viability, cell cycle, and apoptosis, respectively.
RESULTS: We observed increased levels of circ_0072088 and RNF2, and decreased levels of miR-607 in BC tissues. Overexpressing circ_0072088 promoted BC cell proliferation and cell cycle progression while inhibiting apoptosis. Conversely, silencing circ_0072088 had the opposite effects. Our data suggest that circ_0072088 directly targets and downregulates miR-607, which in turn upregulates RNF2, a target of miR-607. Moreover, miR-607 overexpression could mitigate the pro-proliferative and anti-apoptotic effects of circ_0072088 on BC cells.
CONCLUSIONS: Circ_0072088 drives BC progression by downregulating miR-607 and upregulating RNF2, thereby promoting cell proliferation and cycle progression while reducing apoptosis.
摘要：
目的：探讨环状非编码RNAcirc_0072088如何通过影响细胞行为来影响乳腺癌（BC）的进展。
方法：我们测量了circ_0072088，microRNA-607（miR-607）的水平，使用定量实时PCR，BC组织和细胞系中的无名指蛋白2（RNF2）mRNA水平。我们还进行了细胞计数试剂盒-8（CCK-8），BrdU成立，和流式细胞术分析来评估细胞活力，细胞周期,和细胞凋亡，分别。
结果：我们观察到BC组织中circ_0072088和RNF2水平升高，miR-607水平降低。过表达circ_0072088促进BC细胞增殖和细胞周期进程，同时抑制细胞凋亡。相反，沉默circ_0072088具有相反的效果。我们的数据表明，circ_0072088直接靶向并下调miR-607，进而上调miR-607的靶标RNF2。此外，miR-607过表达可以减轻circ_0072088对BC细胞的促增殖和抗凋亡作用。
结论：Circ_0072088通过下调miR-607和上调RNF2来驱动BC进展，从而促进细胞增殖和周期进展，同时减少细胞凋亡。
方法：我们测量了circ_0072088，microRNA-607（miR-607）的水平，使用定量实时PCR，BC组织和细胞系中的无名指蛋白2（RNF2）mRNA水平。我们还进行了细胞计数试剂盒-8（CCK-8），BrdU成立，和流式细胞术分析来评估细胞活力，细胞周期,和细胞凋亡，分别。
结果：我们观察到BC组织中circ_0072088和RNF2水平升高，miR-607水平降低。过表达circ_0072088促进BC细胞增殖和细胞周期进程，同时抑制细胞凋亡。相反，沉默circ_0072088具有相反的效果。我们的数据表明，circ_0072088直接靶向并下调miR-607，进而上调miR-607的靶标RNF2。此外，miR-607过表达可以减轻circ_0072088对BC细胞的促增殖和抗凋亡作用。
结论：Circ_0072088通过下调miR-607和上调RNF2来驱动BC进展，从而促进细胞增殖和周期进展，同时减少细胞凋亡。
