METHODS: We measured the levels of circ_0072088, microRNA-607 (miR-607), and ring finger protein 2 (RNF2) mRNA levels in BC tissues and cell lines using quantitative real-time PCR. We also conducted cell counting kit-8 (CCK-8), BrdU incorporation, and flow cytometry assays to assess cell viability, cell cycle, and apoptosis, respectively.
RESULTS: We observed increased levels of circ_0072088 and RNF2, and decreased levels of miR-607 in BC tissues. Overexpressing circ_0072088 promoted BC cell proliferation and cell cycle progression while inhibiting apoptosis. Conversely, silencing circ_0072088 had the opposite effects. Our data suggest that circ_0072088 directly targets and downregulates miR-607, which in turn upregulates RNF2, a target of miR-607. Moreover, miR-607 overexpression could mitigate the pro-proliferative and anti-apoptotic effects of circ_0072088 on BC cells.
CONCLUSIONS: Circ_0072088 drives BC progression by downregulating miR-607 and upregulating RNF2, thereby promoting cell proliferation and cycle progression while reducing apoptosis.
方法:我们测量了circ_0072088,microRNA-607(miR-607)的水平,使用定量实时PCR,BC组织和细胞系中的无名指蛋白2(RNF2)mRNA水平。我们还进行了细胞计数试剂盒-8(CCK-8),BrdU成立,和流式细胞术分析来评估细胞活力,细胞周期,和细胞凋亡,分别。
结果:我们观察到BC组织中circ_0072088和RNF2水平升高,miR-607水平降低。过表达circ_0072088促进BC细胞增殖和细胞周期进程,同时抑制细胞凋亡。相反,沉默circ_0072088具有相反的效果。我们的数据表明,circ_0072088直接靶向并下调miR-607,进而上调miR-607的靶标RNF2。此外,miR-607过表达可以减轻circ_0072088对BC细胞的促增殖和抗凋亡作用。
结论:Circ_0072088通过下调miR-607和上调RNF2来驱动BC进展,从而促进细胞增殖和周期进展,同时减少细胞凋亡。