Abstract：
OBJECTIVE: To compare differences in euploidy rates for blastocysts in preimplantation genetic testing for aneuploidy (PGT-A) cycles after gonadotropin-releasing hormone agonist (GnRH-a) long and short protocols, GnRH-antagonist (GnRH-ant) protocol, progestin-primed ovarian stimulation and mild stimulation protocols, and other ovary stimulation protocols.
METHODS: This was a retrospective cohort study from the Assisted Reproductive Medicine Department of Shanghai First Maternity and Infant Hospital. A total of 1657 PGT-A cycles with intracytoplasmic sperm injection after different controlled ovary hyperstimulation protocols were analyzed, and a total of 3154 embryos were biopsied. Differences in euploidy rate per embryo biopsied, embryo euploidy rate per oocyte retrieved and cycle cancellation rate were compared.
RESULTS: For the PGT-A cycles, the euploidy rate per embryo biopsied was lower in the GnRH-ant protocol than in the GnRH-a long protocol (53.26 vs. 58.68%, respectively). Multiple linear regression showed that the GnRH-ant protocol was associated with a lower euploidy rate per embryo biopsied (β = -0.079, p = 0.011). The euploidy rate per embryo biopsied was not affected by total gonadotropin dosage, duration of stimulation and number of oocytes retrieved. The embryo euploidy rate per oocyte retrieved was similar in all protocols and was negatively correlated with the total number of oocytes retrieved (β = -0.003, p = 0.003).
CONCLUSIONS: Compared with the GnRH-a long protocol, the GnRH-ant protocol was associated with a lower euploidy rate per embryo biopsied. The total gonadotropin dosage, duration of stimulation and number of oocytes retrieved did not appear to significantly influence euploidy rates.
METHODS: This was a retrospective cohort study from the Assisted Reproductive Medicine Department of Shanghai First Maternity and Infant Hospital. A total of 1657 PGT-A cycles with intracytoplasmic sperm injection after different controlled ovary hyperstimulation protocols were analyzed, and a total of 3154 embryos were biopsied. Differences in euploidy rate per embryo biopsied, embryo euploidy rate per oocyte retrieved and cycle cancellation rate were compared.
RESULTS: For the PGT-A cycles, the euploidy rate per embryo biopsied was lower in the GnRH-ant protocol than in the GnRH-a long protocol (53.26 vs. 58.68%, respectively). Multiple linear regression showed that the GnRH-ant protocol was associated with a lower euploidy rate per embryo biopsied (β = -0.079, p = 0.011). The euploidy rate per embryo biopsied was not affected by total gonadotropin dosage, duration of stimulation and number of oocytes retrieved. The embryo euploidy rate per oocyte retrieved was similar in all protocols and was negatively correlated with the total number of oocytes retrieved (β = -0.003, p = 0.003).
CONCLUSIONS: Compared with the GnRH-a long protocol, the GnRH-ant protocol was associated with a lower euploidy rate per embryo biopsied. The total gonadotropin dosage, duration of stimulation and number of oocytes retrieved did not appear to significantly influence euploidy rates.
摘要：
目的：比较促性腺激素释放激素激动剂（GnRH-a）长和短方案后的非整倍性（PGT-A）周期植入前遗传学测试中胚泡整倍体率的差异，GnRH拮抗剂（GnRH-ant）方案，孕激素引发的卵巢刺激和轻度刺激方案，和其他卵巢刺激方案。
方法：这是上海市第一妇婴医院辅助生殖医学科的一项回顾性队列研究。分析了不同控制性卵巢过度刺激方案后卵胞浆内单精子注射的共1657个PGT-A周期，共对3154个胚胎进行了活检。每个胚胎活检的整倍体率差异，比较了每个卵母细胞的胚胎整倍体率和周期取消率。
结果：对于PGT-A周期，GnRH-ant方案中每个胚胎活检的整倍体率低于GnRH-a长方案(53.26vs.58.68%,分别)。多元线性回归表明，GnRH-ant方案与每个胚胎活检的整倍体率较低相关（β=-0.079，p=0.011）。每个胚胎活检的整倍体率不受总促性腺激素剂量的影响，刺激持续时间和恢复的卵母细胞数量。在所有方案中，每个回收的卵母细胞的胚胎整倍体率相似，并且与回收的卵母细胞总数呈负相关（β=-0.003，p=0.003）。
结论：与GnRH-一个长方案相比，GnRH-ant方案与每个胚胎活检的整倍体率较低相关.促性腺激素的总剂量，刺激持续时间和回收的卵母细胞数量似乎没有显着影响整倍体率。
方法：这是上海市第一妇婴医院辅助生殖医学科的一项回顾性队列研究。分析了不同控制性卵巢过度刺激方案后卵胞浆内单精子注射的共1657个PGT-A周期，共对3154个胚胎进行了活检。每个胚胎活检的整倍体率差异，比较了每个卵母细胞的胚胎整倍体率和周期取消率。
结果：对于PGT-A周期，GnRH-ant方案中每个胚胎活检的整倍体率低于GnRH-a长方案(53.26vs.58.68%,分别)。多元线性回归表明，GnRH-ant方案与每个胚胎活检的整倍体率较低相关（β=-0.079，p=0.011）。每个胚胎活检的整倍体率不受总促性腺激素剂量的影响，刺激持续时间和恢复的卵母细胞数量。在所有方案中，每个回收的卵母细胞的胚胎整倍体率相似，并且与回收的卵母细胞总数呈负相关（β=-0.003，p=0.003）。
结论：与GnRH-一个长方案相比，GnRH-ant方案与每个胚胎活检的整倍体率较低相关.促性腺激素的总剂量，刺激持续时间和回收的卵母细胞数量似乎没有显着影响整倍体率。
